Alloreactive cytotoxic T-cell function, peptide nonspecific
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Mullbacher, Arno; Lobigs, Mario; Kos, Ferdynand; Langman, Rod
Description
The recognition requirements necessary for murine alloreactive cytotoxic T-cells to carry out their effector function has been investigated using target cells that express a unique class I major histocompatibility complex (MHC)-peptide pair. The human cell line T2 and the murine cell line RMA-S are defective in peptide transport components needed to effectively express stable MHC class I molecules at the cell surface. When T2 cells were infected with a vaccinia virus that encoded the K(d) gene...[Show more]
dc.contributor.author | Mullbacher, Arno | |
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dc.contributor.author | Lobigs, Mario | |
dc.contributor.author | Kos, Ferdynand | |
dc.contributor.author | Langman, Rod | |
dc.date.accessioned | 2015-12-13T23:25:01Z | |
dc.identifier.issn | 0300-9475 | |
dc.identifier.uri | http://hdl.handle.net/1885/92487 | |
dc.description.abstract | The recognition requirements necessary for murine alloreactive cytotoxic T-cells to carry out their effector function has been investigated using target cells that express a unique class I major histocompatibility complex (MHC)-peptide pair. The human cell line T2 and the murine cell line RMA-S are defective in peptide transport components needed to effectively express stable MHC class I molecules at the cell surface. When T2 cells were infected with a vaccinia virus that encoded the K(d) gene and provided with a K(d)- motif peptide from the nucleoprotein of influenza virus (NPP), these cells could be lysed by polyclonal allo K(d)-reactive cytotoxic T-lymphocytes (CTL). Similar results were obtained with the murine RMA-S-K(d) cell line, transfected with cDNA able to express some 'empty' K(d) that is heat-labile. Adding another K(d)-motif peptide from influenza virus haemagglutinin (HAP) stabilized the surface expression of K(d) and allowed the RMA-S-K(d) cells to be lysed before or after heat shock. At 27 °C anti-K(d) alloreactive CTL- lysed target cells in the presence and absence of HAP peptide. Alloreactive CTL appear to have a more stringent requirement for a high density of MHC class I on cell surfaces relative to peptide-specific MHC-restricted CTL. We conclude that while K(d)-restricted CTL activity is strictly peptide- specific, anti-K(d)-specific alloreactivity is MHC allele-specific, but peptide-nonspecific. This conclusion is at odds with the Standard Model of T- cell receptor (TCR) function, but consistent with the predictions of a Competing Model of TCR function. | |
dc.publisher | Blackwell Publishing Ltd | |
dc.source | Scandinavian Journal of Immunology | |
dc.subject | Keywords: complementary DNA; major histocompatibility antigen class 1; peptide; t lymphocyte receptor; virus hemagglutinin; alloimmunity; animal cell; antigen expression; controlled study; cytotoxic t lymphocyte; effector cell; heat shock; human; human cell; influe | |
dc.title | Alloreactive cytotoxic T-cell function, peptide nonspecific | |
dc.type | Journal article | |
local.description.notes | Imported from ARIES | |
local.description.refereed | Yes | |
local.identifier.citationvolume | 49 | |
dc.date.issued | 1999 | |
local.identifier.absfor | 110704 - Cellular Immunology | |
local.identifier.ariespublication | MigratedxPub23604 | |
local.type.status | Published Version | |
local.contributor.affiliation | Mullbacher, Arno, College of Medicine, Biology and Environment, ANU | |
local.contributor.affiliation | Lobigs, Mario, College of Medicine, Biology and Environment, ANU | |
local.contributor.affiliation | Kos, Ferdynand, La Jolla Institute for Allergy and Immunology | |
local.contributor.affiliation | Langman, Rod, Salk Institute | |
local.description.embargo | 2037-12-31 | |
local.bibliographicCitation.startpage | 563 | |
local.bibliographicCitation.lastpage | 569 | |
local.identifier.doi | 10.1046/j.1365-3083.1999.00568.x | |
dc.date.updated | 2015-12-12T09:23:04Z | |
local.identifier.scopusID | 2-s2.0-0003399462 | |
Collections | ANU Research Publications |
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