Changes in Chromatin Accessibility Across the GM-CSF Promoter upon T Cell Activation Are Dependent on Nuclear Factor kappaB Proteins
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Holloway, Adele; Rao, Sudha; Chen, Xin; Shannon, M Frances
Description
Granulocyte/macrophage colony-stimulating factor (GM-CSF) is a key cytokine in myelopoiesis and aberrant expression is associated with chronic inflammatory disease and myeloid leukemias. This aberrant expression is often associated with constitutive nuclear factor (NF)-κB activation. To investigate the relationship between NF-κB and GM-CSF transcription in a chromatin context, we analyzed the chromatin structure of the GM-CSF gene in T cells and the role of NF-κB proteins in chromatin...[Show more]
dc.contributor.author | Holloway, Adele | |
---|---|---|
dc.contributor.author | Rao, Sudha | |
dc.contributor.author | Chen, Xin | |
dc.contributor.author | Shannon, M Frances | |
dc.date.accessioned | 2015-12-13T23:24:41Z | |
dc.identifier.issn | 0022-1007 | |
dc.identifier.uri | http://hdl.handle.net/1885/92330 | |
dc.description.abstract | Granulocyte/macrophage colony-stimulating factor (GM-CSF) is a key cytokine in myelopoiesis and aberrant expression is associated with chronic inflammatory disease and myeloid leukemias. This aberrant expression is often associated with constitutive nuclear factor (NF)-κB activation. To investigate the relationship between NF-κB and GM-CSF transcription in a chromatin context, we analyzed the chromatin structure of the GM-CSF gene in T cells and the role of NF-κB proteins in chromatin remodeling. We show here that chromatin remodeling occurs across a region of the GM-CSF gene between -174 and +24 upon T cell activation, suggesting that remodeling is limited to a single nucleosome encompassing the proximal promoter. Nuclear NF-κB levels appear to play a critical role in this process. In addition, using an immobilized template assay we found that the ATPase component of the SWI/SNF chromatin remodeling complex, brg1, is recruited to the GM-CSF proximal promoter in an NF-κB-dependent manner in vitro. These results suggest that chromatin remodeling across the GM-CSF promoter in T cells is a result of recruitment of SWI/SNF type remodeling complexes by NF-κB proteins binding to the CD28 response region of the promoter. | |
dc.publisher | Rockefeller University Press | |
dc.source | Journal of Experimental Medicine | |
dc.subject | Keywords: adenosine triphosphatase; CD28 antigen; granulocyte macrophage colony stimulating factor; immunoglobulin enhancer binding protein; transcription factor; transcription factor Brg1; unclassified drug; animal cell; article; cell level; chromatin structure; c Brg1; Chromatin remodeling; Cytokine; Re1A; Transcription | |
dc.title | Changes in Chromatin Accessibility Across the GM-CSF Promoter upon T Cell Activation Are Dependent on Nuclear Factor kappaB Proteins | |
dc.type | Journal article | |
local.description.notes | Imported from ARIES | |
local.description.refereed | Yes | |
local.identifier.citationvolume | 197 | |
dc.date.issued | 2003 | |
local.identifier.absfor | 060111 - Signal Transduction | |
local.identifier.ariespublication | MigratedxPub23392 | |
local.type.status | Published Version | |
local.contributor.affiliation | Holloway, Adele, College of Medicine, Biology and Environment, ANU | |
local.contributor.affiliation | Rao, Sudha, College of Medicine, Biology and Environment, ANU | |
local.contributor.affiliation | Chen, Xin, College of Medicine, Biology and Environment, ANU | |
local.contributor.affiliation | Shannon, M Frances, College of Medicine, Biology and Environment, ANU | |
local.description.embargo | 2037-12-31 | |
local.bibliographicCitation.issue | 4 | |
local.bibliographicCitation.startpage | 413 | |
local.bibliographicCitation.lastpage | 423 | |
local.identifier.doi | 10.1084/jem.20021039 | |
dc.date.updated | 2015-12-12T09:21:26Z | |
local.identifier.scopusID | 2-s2.0-0037450739 | |
Collections | ANU Research Publications |
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