Laver, Derek Rowland; Eager, K; Taoube, L; Lamb, Graham
Ryanodine receptor (RyR)-Ca2+ release channels from rabbit skeletal muscle were incorporated into lipid bilayers. The effects of cytoplasmic and luminal pH were studied separately over the pH range 5-8, using half-unit intervals. RyR activity (at constant luminal pH of 7.5) was inhibited at acidic cytoplasmic pH, with a half-inhibitory pH (PH1) ~6.5, irrespective of bilayer potential and of whether the RyRs were activated by cytoplasmic Ca2+ (50 μM), ATP (2 or 5 mM), or both. Inhibition...[Show more]
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