The complete cDNA Sequences of IL-2, IL-4, IL-6 and IL-10 from the European rabbit (oryctolagus cuniculus)
Date
2000
Authors
Perkins, Harvey
van Leeuwen, Barbara
Hardy, Chris M
Kerr, P J
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Volume Title
Publisher
Academic Press
Abstract
The cDNAs for four rabbit cytokine genes [interleukin 2 (IL-2), IL-4, IL-6 and IL-10] have been cloned from primary lymphocytes by polymerase chain reaction (PCR) methods. IL-2 and IL-10 are both highly conserved between rabbit and other species, IL-4 and IL-6 are less strongly conserved, at both nucleotide and amino acid levels, and exhibit structural differences. An extension of the coding region of rabbit IL-6 relative to all other reported IL-6 genes results from a mutation in the usual stop codon which allows translation to continue for a further 27 amino acids. Analysis of IL-6 from four other lagomorph species suggests that this mutation is specific to the European rabbit. Sequence and structural differences of IL-4 and IL-6, while presumably not altering function, may render them highly species-specific. Several alternatively spliced variants of IL-2 and IL-4 are also reported. (C) 2000 Academic Press.
Description
Keywords
Keywords: complementary DNA; interleukin 10; interleukin 2; interleukin 4; interleukin 6; article; DNA sequence; molecular cloning; nonhuman; nucleotide sequence; polymerase chain reaction; priority journal; rabbit; stop codon; Amino Acid Sequence; Animals; Cats; C Alternative splicing; Interleukin; Lagomorph; Rabbit
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Source
Cytokine
Type
Journal article
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2037-12-31