Proteome analysis of male gametophyte development in rice anthers
Date
2003
Authors
Kerim, Tursun
Imin, Nijat
Weinman, Jeremy
Rolfe, Barry
Journal Title
Journal ISSN
Volume Title
Publisher
Wiley-VCH Verlag GMBH
Abstract
We used proteomic analysis to investigate the changing patterns of protein synthesis during pollen development in anthers from rice plants grown under strictly controlled growth conditions. Cytological analysis and external growth measurements such as anther length, auricle distances and days before flowering were used to determine pollen developmental stages. This allowed the collection of synchronous anther materials representing six discrete pollen developmental stages. Proteins were extracted from the anther samples and separated by two-dimensional gel electrophoresis to produce proteome maps. The anther proteome maps of different developmental stages were compared and 150 protein spots, which were changed consistently during development, were analysed by matrix-assisted laser desorption/ionization-time of flight mass spectrometry to produce peptide mass fingerprint (PMF) data. Database searches using these PMF data revealed the identities of 40 of the protein spots analyzed. These 40 proteins represent 33 unique gene products. Four protein spots that could not be identified by PMF analysis were analysed by N-terminal microsequencing. Multiple charge-isoforms of vacuolar acid invertase, fructokinase, β-expansin and profilin were identified. These proteins are closely associated with sugar metabolism, cell elongation and cell expansion, all of which are cell activities that are essential to pollen germination. The existence of multiple isoforms of the same proteins suggests that during the process of pollen development some kind of post-translational modification of these proteins occurs.
Description
Keywords
Keywords: beta fructofuranosidase; fructokinase; patulin; profilin; amino acid sequence; amino terminal sequence; anther; article; carbohydrate metabolism; cell growth; cell kinetics; controlled study; cytology; developmental biology; developmental stage; ear; game Microspore development; Peptide mass fingerprinting-; Rice (Oryza sativa L.); Two-dimensional gel electrophoresis
Citation
Collections
Source
Proteomics
Type
Journal article