An XX/XY sex microchromosome system in a freshwater turtle, Chelodina longicollis (Testudines: Chelidae) with genetic sex determination
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Ezaz, Tariq; Valenzuela, Nicole; Gruetzner, Frank; Miura, Ikuo; Georges, Arthur; Burke, Russell L; Graves, Jennifer
Description
Heteromorphic sex chromosomes are rare in turtles, having been described in only four species. Like many turtle species, the Australian freshwater turtle Chelodina longicollis has genetic sex determination, but no distinguishable (heteromorphic) sex chromosomes were identified in a previous karyotyping study. We used comparative genomic hybridization (CGH) to show that C. longicollis has an XX/XY system of chromosomal sex determination, involving a pair of microchromosomes. C-banding and...[Show more]
dc.contributor.author | Ezaz, Tariq | |
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dc.contributor.author | Valenzuela, Nicole | |
dc.contributor.author | Gruetzner, Frank | |
dc.contributor.author | Miura, Ikuo | |
dc.contributor.author | Georges, Arthur | |
dc.contributor.author | Burke, Russell L | |
dc.contributor.author | Graves, Jennifer | |
dc.date.accessioned | 2015-12-13T23:04:14Z | |
dc.identifier.issn | 0967-3849 | |
dc.identifier.uri | http://hdl.handle.net/1885/85277 | |
dc.description.abstract | Heteromorphic sex chromosomes are rare in turtles, having been described in only four species. Like many turtle species, the Australian freshwater turtle Chelodina longicollis has genetic sex determination, but no distinguishable (heteromorphic) sex chromosomes were identified in a previous karyotyping study. We used comparative genomic hybridization (CGH) to show that C. longicollis has an XX/XY system of chromosomal sex determination, involving a pair of microchromosomes. C-banding and reverse fluorescent staining also distinguished microchromosomes with different banding patterns in males and females in ∼70% cells examined. GTG-banding did not reveal any heteromorphic chromosomes, and no replication asynchrony on the X or Y microchromosomes was observed using replication banding. We conclude that there is a very small sequence difference between X and Y chromosomes in this species, a difference that is consistently detectable only by high-resolution molecular cytogenetic techniques, such as CGH. This is the first time a pair of microchromosomes has been identified as the sex chromosomes in a turtle species. | |
dc.publisher | Kluwer Academic Publishers | |
dc.source | Chromosome Research | |
dc.subject | Keywords: fluorescent dye; animal cell; animal experiment; article; C banding; chromosome high resolution banding analysis; chromosome replication; comparative genomic hybridization; controlled study; female; gender and sex; genetics; karyotype 46,XX; karyotype 46, CGH; Chelodina longicollis; Comparative genomic hybridization; Eastern snake-necked turtle; Microchromosomes; Sex chromosomes | |
dc.title | An XX/XY sex microchromosome system in a freshwater turtle, Chelodina longicollis (Testudines: Chelidae) with genetic sex determination | |
dc.type | Journal article | |
local.description.notes | Imported from ARIES | |
local.description.refereed | Yes | |
local.identifier.citationvolume | 14 | |
dc.date.issued | 2006 | |
local.identifier.absfor | 060403 - Developmental Genetics (incl. Sex Determination) | |
local.identifier.ariespublication | MigratedxPub13599 | |
local.type.status | Published Version | |
local.contributor.affiliation | Ezaz, Tariq, College of Medicine, Biology and Environment, ANU | |
local.contributor.affiliation | Valenzuela, Nicole, Iowa State University | |
local.contributor.affiliation | Gruetzner, Frank, College of Medicine, Biology and Environment, ANU | |
local.contributor.affiliation | Miura, Ikuo, Hiroshima University | |
local.contributor.affiliation | Georges, Arthur, University of Canberra | |
local.contributor.affiliation | Burke, Russell L, Hofstra University | |
local.contributor.affiliation | Graves, Jennifer, College of Medicine, Biology and Environment, ANU | |
local.description.embargo | 2037-12-31 | |
local.bibliographicCitation.issue | 2 | |
local.bibliographicCitation.startpage | 139 | |
local.bibliographicCitation.lastpage | 150 | |
local.identifier.doi | 10.1007/s10577-006-1029-6 | |
dc.date.updated | 2015-12-12T07:54:14Z | |
local.identifier.scopusID | 2-s2.0-33644962512 | |
Collections | ANU Research Publications |
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