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Translational incorporation of L-3,4-dihydroxyphenylalanine into proteins

Ozawa, Kiyoshi; Headlam, Madeleine; Mouradov, Dmitri; Watt, Stephen J; Beck, Jennifer; Rodgers, Kenneth; Dean, Roger; Dixon, Nicholas; Otting, Gottfried; Huber, Thomas

Description

An Escherichia coli cell-free transcription/translation system was used to explore the high-level incorporation of L-3,4-dihydroxyphenylalanine (DOPA) into proteins by replacing tyrosine with DOPA in the reaction mixtures. ESI-MS showed specific incorporation of DOPA in place of tyrosine. More than 90% DOPA incorporation at each tyrosine site was achieved, allowing the recording of clean15N-HSQC NMR spectra. A redox-staining method specific for DOPA was shown to provide a sensitive and...[Show more]

CollectionsANU Research Publications
Date published: 2005
Type: Journal article
URI: http://hdl.handle.net/1885/78854
Source: The FEBS Journal
DOI: 10.1111/j.1742-4658.2005.04735.x

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