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FRAP analysis of nucleocytoplasmic dynamics of the vitamin D receptor splice variant VDRB1: preferential targeting to nuclear speckles

Sunn, Kathryn L; Eisman, John A; Gardiner, Edith M; Jans, David A

Description

Although the key components of the cellular nuclear transport machinery have largely been characterized through extensive efforts in recent years, in vivo measurements of the kinetics of nuclear protein import/export are patently few. The present study applies the approach of FRAP (fluorescence recovery after photobleaching) to examine the nucleocytoplasmic flux of a novel human VDRB1 (vitamin D receptor B1) isoform in living cells. Through an N-terminal extension containing a consensus nuclear...[Show more]

dc.contributor.authorSunn, Kathryn L
dc.contributor.authorEisman, John A
dc.contributor.authorGardiner, Edith M
dc.contributor.authorJans, David A
dc.date.accessioned2015-12-13T22:35:22Z
dc.identifier.issn0264-6021
dc.identifier.urihttp://hdl.handle.net/1885/76556
dc.description.abstractAlthough the key components of the cellular nuclear transport machinery have largely been characterized through extensive efforts in recent years, in vivo measurements of the kinetics of nuclear protein import/export are patently few. The present study applies the approach of FRAP (fluorescence recovery after photobleaching) to examine the nucleocytoplasmic flux of a novel human VDRB1 (vitamin D receptor B1) isoform in living cells. Through an N-terminal extension containing a consensus nuclear targeting sequence, VDRB1 is capable of localizing in nuclear speckles adjacent to SC-35 (35 kDa splicing component)- containing speckles as well as in the nucleoplasm, dependent on ligand. Investigation of VDRB1 nucleocytoplasmic transport using FRAP indicates for the first time that the VDRB1 has a serum-modulated, active nuclear import mechanism. There is no evidence of an efficient, active export mechanism for VDRB1, probably as a result of nuclear retention. VDRB1 nuclear import in the absence of serum occurred more rapidly and to a greater extent to nuclear speckles compared with import to other nuclear sites. This preferential transport from the cytoplasm to and accumulation within nuclear speckles is consistent with the idea that the latter represent dynamic centres of VDRB1 interaction with other nuclear proteins. The results are consistent with the existence of specialized pathways to target proteins to nuclear subdomains.
dc.publisherPortland Press
dc.sourceBiochemical Journal
dc.subjectKeywords: Cytology; Fluorescence; Proteins; Vitamins; Living cells; Nuclear proteins; Nucleocytoplasmic dynamics; Serums; Cells; ligand; nuclear protein; unclassified drug; vitamin D receptor; vitamin d receptor b1; animal cell; article; cell transport; cytoplasm; Fluorescence recovery after photobleaching (FRAP); Nuclear export; Nuclear hormone receptor; Nuclear speckle; Nucleus; Vitamin D receptor B1 (VDRB1)
dc.titleFRAP analysis of nucleocytoplasmic dynamics of the vitamin D receptor splice variant VDRB1: preferential targeting to nuclear speckles
dc.typeJournal article
local.description.notesImported from ARIES
local.description.refereedYes
local.identifier.citationvolume388
dc.date.issued2005
local.identifier.absfor060111 - Signal Transduction
local.identifier.ariespublicationMigratedxPub5376
local.type.statusPublished Version
local.contributor.affiliationSunn, Kathryn L, Garvan Institute of Medical Research
local.contributor.affiliationEisman, John A, Garvan Institute of Medical Research
local.contributor.affiliationGardiner, Edith M, Garvan Institute of Medical Research
local.contributor.affiliationJans, David A, College of Medicine, Biology and Environment, ANU
local.description.embargo2037-12-31
local.bibliographicCitation.startpage509
local.bibliographicCitation.lastpage514
local.identifier.doi10.1042/BJ20042040
dc.date.updated2015-12-11T09:27:28Z
local.identifier.scopusID2-s2.0-20544465655
CollectionsANU Research Publications

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