On the evolution and molecular epidemiology of the potyvirus Papaya ringspot virus
Date
2002
Authors
Bateson, Marion
Lines, Rosemarie
Revill, Peter
Chaleeprom, Worawan
Ha, Cuong
Gibbs, Adrian
Dale, James
Journal Title
Journal ISSN
Volume Title
Publisher
Society for General Microbiology
Abstract
The potyvirus Papaya ringspot virus (PRSV) is found throughout the tropics and subtropics. Its P biotype is a devastating pathogen of papaya crops and its W biotype of cucurbits. PRSV-P is thought to arise by mutation from PRSV-W. However, the relative impact of mutation and movement on the structure of PRSV populations is not well characterized. To investigate this, we have determined the coat protein sequences of isolates of both biotypes of PRSV from Vietnam (50), Thailand (13), India (1) and the Philippines (1), and analysed them together with 28 PRSV sequences already published, so that we can better understand the molecular epidemiology and evolution of PRSV. In Thailand, variation was greater among PRSV-W isolates (mean nucleotide divergence 7.6%) than PRSV-P isolates (mean 2.6%), but in Vietnamese populations the P and W biotypes were more but similarly diverse. Phylogenetic analyses of PRSV also involving its closest known relative, Moroccan watermelon mosaic virus, indicate that PRSV may have originated in Asia, particularly in the Indian subcontinent, as PRSV populations there are most diverse and hence have probably been present longest. Our analyses show that mutation, together with local and long-distance movement, contributes to population variation, and also confirms an earlier conclusion that populations of the PRSV-P biotype have evolved on several occasions from PRSV-W populations.
Description
Keywords
Keywords: coat protein; amino acid sequence; article; Asia; biotype; controlled study; crop; cucumber; epidemiology; gene mutation; India; molecular evolution; Mosaic virus; nonhuman; nucleotide sequence; papaya virus; Philippines; phylogeny; Potyvirus; priority jo
Citation
Collections
Source
Journal of General Virology
Type
Journal article