An intradermal model for vaccinia virus pathogenesis in mice
Date
2012
Authors
Lin, Chien-Wei (Leon)
Smith, Stewart
Tscharke, David
Journal Title
Journal ISSN
Volume Title
Publisher
Humana Press
Abstract
Intradermal injection of vaccinia virus in the ear pinnae of mice provides a model of dermal infection and vaccination. The key features of this model are the appearance of a lesion on the surface of the ear that can be measured as a clinical sign of disease and substantial growth of virus in the infected skin in the absence of systemic spread. In addition, infected ears can be easily removed to allow virological, histological, and cellular analyses. Finally, evaluation of the roles of virus (and presumably also host) genes in vaccinia virus pathogenesis in the intradermal model can yield different results than similar experiments using other routes and may reveal otherwise unknown functions.
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Keywords
Keywords: animal; article; Bagg albino mouse; C57BL mouse; disease model; external ear; host pathogen interaction; intradermal drug administration; mouse; pathology; physiology; skin infection; vaccinia; Vaccinia virus; virology; virus load; Animals; Disease Models
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Source
Type
Book chapter
Book Title
Vaccinia Virus and Poxvirology. Methods and Protocols
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Restricted until
2037-12-31
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