Development of an allele-specific PCR for Escherichia coli B2 sub-typing, a rapid and easy to perform substitute of multilocus sequence typing
Date
2014
Authors
Clermont, Olivier
Christenson, Julia
Daubie, Anne-Sophie
Gordon, David
Denamur, Erick
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Elsevier
Abstract
We developed and validated an allele-specific PCR method for detecting the nine main Escherichia coli phylogroup B2 lineages involved in extra-intestinal infections, which could be used as a substitute for multilocus sequence typing in studies for which the greater resolution at the sequence type level is not needed.
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Journal of Microbiological Methods
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Journal article
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2037-12-31
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