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Biofilm Formation by and Thermal Niche and Virulence Characteristics of Escherichia spp.

Ingle, Danielle; Clermont, Olivier; Skurnik, David; Denamur, Erick; Walk, Seth T.; Gordon, David

Description

In order to better understand the ecological and virulence characteristics of the various clades of Escherichia, in vitro and in vivo experiments were undertaken. Members of the recently described cryptic clades of Escherichia (clades III, IV, and V) were found to have an enhanced ability to form biofilms compared to strains of Escherichia coli, E. fergusonii, or E. albertii. Members of the cryptic clades were also able to replicate at a lower temperature (5°C versus 11°C) than strains of the...[Show more]

dc.contributor.authorIngle, Danielle
dc.contributor.authorClermont, Olivier
dc.contributor.authorSkurnik, David
dc.contributor.authorDenamur, Erick
dc.contributor.authorWalk, Seth T.
dc.contributor.authorGordon, David
dc.date.accessioned2015-12-10T23:07:03Z
dc.identifier.issn0099-2240
dc.identifier.urihttp://hdl.handle.net/1885/62923
dc.description.abstractIn order to better understand the ecological and virulence characteristics of the various clades of Escherichia, in vitro and in vivo experiments were undertaken. Members of the recently described cryptic clades of Escherichia (clades III, IV, and V) were found to have an enhanced ability to form biofilms compared to strains of Escherichia coli, E. fergusonii, or E. albertii. Members of the cryptic clades were also able to replicate at a lower temperature (5°C versus 11°C) than strains of the named species of Escherichia. Neither a strain's maximal growth rate nor its optimal temperature for growth varied with respect to the strain's phylogenetic affiliation. Escherichia strains not belonging to the species E. coli were positive for a mix of traits thought to enhance a strain's ability to cause either intestinal or extraintestinal disease. However, no non-E. coli Escherichia strain was virulent in a mouse model of extraintestinal infection. The frequency of resistance to antibiotics was low, and none of the strains tested harbored class 1, 2, or 3 integrons. The results of these experiments support the hypothesis that members of the cryptic Escherichia clades may be better able to persist in the external environment compared to E. coli, E. fergusonii, or E. albertii, isolates.
dc.publisherAmerican Society for Microbiology
dc.rightsAuthor/s retain copyright
dc.sourceApplied and Environmental Microbiology
dc.subjectKeywords: Biofilm formation; Class 1; E. coli; External environments; In-vitro; Integrons; Mouse models; Optimal temperature; Phylogenetic affiliation; Resistance to antibiotics; Biofilms; Ecology; Escherichia coli; Experiments; Strain rate; antibiotics; biofilm; c
dc.titleBiofilm Formation by and Thermal Niche and Virulence Characteristics of Escherichia spp.
dc.typeJournal article
local.description.notesImported from ARIES
local.identifier.citationvolume77
dc.date.issued2011
local.identifier.absfor060504 - Microbial Ecology
local.identifier.ariespublicationu9511635xPUB750
local.type.statusPublished Version
local.contributor.affiliationIngle, Danielle, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationClermont, Olivier, Universite Paris
local.contributor.affiliationSkurnik, David, Universite Paris Diderot
local.contributor.affiliationDenamur, Erick, Universite Paris
local.contributor.affiliationWalk, Seth T., University of Michigan Health System
local.contributor.affiliationGordon, David, College of Medicine, Biology and Environment, ANU
local.bibliographicCitation.issue8
local.bibliographicCitation.startpage2695
local.bibliographicCitation.lastpage2700
local.identifier.doi10.1128/AEM.02401-10
local.identifier.absseo970106 - Expanding Knowledge in the Biological Sciences
dc.date.updated2016-02-24T12:08:14Z
local.identifier.scopusID2-s2.0-79955561849
local.identifier.thomsonID000289459300017
dcterms.accessRightsOpen Access
CollectionsANU Research Publications

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