Spectroscopic selection of distance measurements in a protein dimer with mixed nitroxide and Gd 3+ spin labels
The pulse DEER (Double Electron-Electron Resonance) technique is frequently applied for measuring nanometer distances between specific sites in biological macromolecules. In this work we extend the applicability of this method to high field distance measurements in a protein assembly with mixed spin labels, i.e. a nitroxide spin label and a Gd 3+ tag. We demonstrate the possibility of spectroscopic selection of distance distributions between two nitroxide spin labels, a nitroxide spin label and...[Show more]
|Collections||ANU Research Publications|
|Source:||Physical Chemistry Chemical Physics|
|01_Kaminker_Spectroscopic_selection_of_2012.pdf||1.36 MB||Adobe PDF||Request a copy|
Items in Open Research are protected by copyright, with all rights reserved, unless otherwise indicated.