Thiol-ene reaction: a versatile tool in site-specific labelling of proteins with chemically inert tags for paramagnetic NMR
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Li, Qing-Feng; Yang, Yin; Maleckis, Ansis; Su, Xun-Cheng; Otting, Gottfried
Description
Site-specific tagging of proteins with paramagnetic lanthanides generates valuable long-range structure restraints for structural biology by NMR spectroscopy. We show that the thiol-ene addition reaction offers a powerful tool for tagging proteins in a chemically stable manner with very small lanthanide tags. This journal is
Collections | ANU Research Publications |
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Date published: | 2012 |
Type: | Journal article |
URI: | http://hdl.handle.net/1885/60820 |
Source: | Chemical Communications |
DOI: | 10.1039/c2cc17900h |
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01_Li_Thiol-ene_reaction:_a_2012.pdf | 1.26 MB | Adobe PDF | Request a copy |
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