Thiol-ene reaction: a versatile tool in site-specific labelling of proteins with chemically inert tags for paramagnetic NMR
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Li, Qing-Feng; Yang, Yin; Maleckis, Ansis; Su, Xun-Cheng; Otting, Gottfried
Description
Site-specific tagging of proteins with paramagnetic lanthanides generates valuable long-range structure restraints for structural biology by NMR spectroscopy. We show that the thiol-ene addition reaction offers a powerful tool for tagging proteins in a chemically stable manner with very small lanthanide tags. This journal is
dc.contributor.author | Li, Qing-Feng | |
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dc.contributor.author | Yang, Yin | |
dc.contributor.author | Maleckis, Ansis | |
dc.contributor.author | Su, Xun-Cheng | |
dc.contributor.author | Otting, Gottfried | |
dc.date.accessioned | 2015-12-10T22:58:21Z | |
dc.identifier.issn | 1359-7345 | |
dc.identifier.uri | http://hdl.handle.net/1885/60820 | |
dc.description.abstract | Site-specific tagging of proteins with paramagnetic lanthanides generates valuable long-range structure restraints for structural biology by NMR spectroscopy. We show that the thiol-ene addition reaction offers a powerful tool for tagging proteins in a chemically stable manner with very small lanthanide tags. This journal is | |
dc.publisher | Royal Society of Chemistry | |
dc.source | Chemical Communications | |
dc.subject | Keywords: cysteine; dipicolinic acid; iodoacetamide; lanthanide; maleimide derivative; oxygen; peptidylprolyl isomerase; protein tag; sulfide; ubiquitin; addition reaction; alpha helix; amino acid sequence; article; chirality; crystal structure; heteronuclear singl | |
dc.title | Thiol-ene reaction: a versatile tool in site-specific labelling of proteins with chemically inert tags for paramagnetic NMR | |
dc.type | Journal article | |
local.description.notes | Imported from ARIES | |
local.identifier.citationvolume | 48 | |
dc.date.issued | 2012 | |
local.identifier.absfor | 030403 - Characterisation of Biological Macromolecules | |
local.identifier.ariespublication | u4005981xPUB566 | |
local.type.status | Published Version | |
local.contributor.affiliation | Li, Qing-Feng, Nankai University | |
local.contributor.affiliation | Yang, Yin, Nankai University | |
local.contributor.affiliation | Maleckis, Ansis, College of Physical and Mathematical Sciences, ANU | |
local.contributor.affiliation | Otting, Gottfried, College of Physical and Mathematical Sciences, ANU | |
local.contributor.affiliation | Su, Xun-Cheng, College of Physical and Mathematical Sciences, ANU | |
local.description.embargo | 2037-12-31 | |
local.bibliographicCitation.issue | 21 | |
local.bibliographicCitation.startpage | 2704 | |
local.bibliographicCitation.lastpage | 2706 | |
local.identifier.doi | 10.1039/c2cc17900h | |
local.identifier.absseo | 970106 - Expanding Knowledge in the Biological Sciences | |
dc.date.updated | 2016-02-24T10:24:48Z | |
local.identifier.scopusID | 2-s2.0-84863115471 | |
local.identifier.thomsonID | 000300174100016 | |
Collections | ANU Research Publications |
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