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Thiol-ene reaction: a versatile tool in site-specific labelling of proteins with chemically inert tags for paramagnetic NMR

Li, Qing-Feng; Yang, Yin; Maleckis, Ansis; Su, Xun-Cheng; Otting, Gottfried

Description

Site-specific tagging of proteins with paramagnetic lanthanides generates valuable long-range structure restraints for structural biology by NMR spectroscopy. We show that the thiol-ene addition reaction offers a powerful tool for tagging proteins in a chemically stable manner with very small lanthanide tags. This journal is

dc.contributor.authorLi, Qing-Feng
dc.contributor.authorYang, Yin
dc.contributor.authorMaleckis, Ansis
dc.contributor.authorSu, Xun-Cheng
dc.contributor.authorOtting, Gottfried
dc.date.accessioned2015-12-10T22:58:21Z
dc.identifier.issn1359-7345
dc.identifier.urihttp://hdl.handle.net/1885/60820
dc.description.abstractSite-specific tagging of proteins with paramagnetic lanthanides generates valuable long-range structure restraints for structural biology by NMR spectroscopy. We show that the thiol-ene addition reaction offers a powerful tool for tagging proteins in a chemically stable manner with very small lanthanide tags. This journal is
dc.publisherRoyal Society of Chemistry
dc.sourceChemical Communications
dc.subjectKeywords: cysteine; dipicolinic acid; iodoacetamide; lanthanide; maleimide derivative; oxygen; peptidylprolyl isomerase; protein tag; sulfide; ubiquitin; addition reaction; alpha helix; amino acid sequence; article; chirality; crystal structure; heteronuclear singl
dc.titleThiol-ene reaction: a versatile tool in site-specific labelling of proteins with chemically inert tags for paramagnetic NMR
dc.typeJournal article
local.description.notesImported from ARIES
local.identifier.citationvolume48
dc.date.issued2012
local.identifier.absfor030403 - Characterisation of Biological Macromolecules
local.identifier.ariespublicationu4005981xPUB566
local.type.statusPublished Version
local.contributor.affiliationLi, Qing-Feng, Nankai University
local.contributor.affiliationYang, Yin, Nankai University
local.contributor.affiliationMaleckis, Ansis, College of Physical and Mathematical Sciences, ANU
local.contributor.affiliationOtting, Gottfried, College of Physical and Mathematical Sciences, ANU
local.contributor.affiliationSu, Xun-Cheng, College of Physical and Mathematical Sciences, ANU
local.description.embargo2037-12-31
local.bibliographicCitation.issue21
local.bibliographicCitation.startpage2704
local.bibliographicCitation.lastpage2706
local.identifier.doi10.1039/c2cc17900h
local.identifier.absseo970106 - Expanding Knowledge in the Biological Sciences
dc.date.updated2016-02-24T10:24:48Z
local.identifier.scopusID2-s2.0-84863115471
local.identifier.thomsonID000300174100016
CollectionsANU Research Publications

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