A novel pair of universal primers for the detection of potyviruses
A novel pair of universal primers was developed to detect potyvirus species after conserved sites were identified using all full-length potyvirus sequences available by 2005. The breadth of specificity of the new primers, NIb2F and NIb3R, was investigated and compared with the specificity of two routinely used primer pairs in plant virus diagnostic laboratories. RNA from 40 potyvirus isolates representing 23 recognized and three possible new species was tested. Reactions with NIb2F and NIb3R...[Show more]
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