Salis, Andrea; Bostrom, Mathias; Medda, Luca; Cugia, Francesca; Barse, Brajesh; Parsons, Drew; Ninham, Barry; Monduzzi, Maura
The points of zero charge/potential of proteins depend not only on pH but also on how they are measured. They depend also on background salt solution type and concentration. The protein isoelectric point (IEP) is determined by electrokinetical measurements, whereas the isoionic point (IIP) is determined by potentiometric titrations. Here we use potentiometric titration and zeta potential (Χ) measurements at different NaCl concentrations to study systematically the effect of ionic strength on...[Show more]
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