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Characterization of the Secretome of Suspension Cultures of Medicago Species Reveals Proteins Important for Defense and Development

Kusumawati, Lucia; Imin, Nijat; Djordjevic, Michael


Molecular events occurring in the plant apoplast contribute to important developmental and defense responses. To define the secretome of Medicago, we used suspension cultures to isolate and identify secreted proteins as a first step to determining their functions. Proteins in the extracellular medium of the suspension cultures were examined using SDS-PAGE, tandem mass spectrometry (MALDI-TOF/TOF) and bioinformatics tools. There were 39 proteins identified in the cultures derived from M. sativa,...[Show more]

CollectionsANU Research Publications
Date published: 2008
Type: Journal article
Source: Journal of Proteome Research
DOI: 10.1021/pr800291z


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