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Analysis of anabolic steroids in the horse: development of a generic ELISA for the screening of 17α-alkyl anabolic steroid metabolites

Hungerford, Natasha; Sortais, Benoit; Smart, Corrine G; McKinney, Andrew; Ridley, Damon D; Stenhouse, Allen M; Suann, Craig J; Munn, Kellie J; Sillence, Martin N; McLeod, Malcolm

Description

Due to the potential for misuse of a wide range of anabolic steroids in horse racing, a screening test to detect multiple compounds, via a common class of metabolites, would be a valuable forensic tool. An enzyme-linked immunosorbent assay (ELISA) has been developed to detect 17α-alkyl anabolic steroid metabolites in equine urine. 16β-Hydroxymestanolone (16β,17β-dihydroxy-17α-methyl-5α-androstan-3-one) was synthesised in six steps from commercially available epiandrosterone...[Show more]

CollectionsANU Research Publications
Date published: 2005
Type: Journal article
URI: http://hdl.handle.net/1885/53023
Source: Journal of Steroid Biochemistry and Molecular Biology
DOI: 10.1016/j.jsbmb.2005.03.007

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