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Resistance to Wheat streak mosaic virus generated by expression of an artificial polycistronic microRNA in wheat

Fahim, Muhammad; Millar, Anthony; Wood, Craig C; Larkin , Phillip J

Description

Wheat streak mosaic virus (WSMV) is a persistent threat to wheat production, necessitating novel approaches for protection. We developed an artificial miRNA strategy against WSMV, incorporating five amiRNAs within one polycistronic amiRNA precursor. Using miRNA sequence and folding rules, we chose five amiRNAs targeting conserved regions of WSMV but avoiding off-targets in wheat. These replaced the natural miRNA in each of five arms of the polycistronic rice miR395, producing amiRNA precursor,...[Show more]

CollectionsANU Research Publications
Date published: 2012
Type: Journal article
URI: http://hdl.handle.net/1885/50470
Source: Plant Biotechnology Journal
DOI: 10.1111/j.1467-7652.2011.00647.x

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