Proteomic analysis of temperature stress in plants
Date
2010
Authors
Neilson , Karlie
Gammulla, C. Gayani
Mirzaei, Mehdi
Imin, Nijat
Haynes, Paul
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Publisher
Wiley-VCH Verlag GMBH
Abstract
In this review we examine current approaches used for proteomic analysis of temperature stress in plants. Rapid advances in this field in recent years are discussed, including metabolic, chemical and isotopic labeling, and label-free quantitative techniques. These are compared and contrasted with well-established methods such as 2-DE approaches. Examples of applications of various methods are presented, and technical difficulties and limitations of each are also considered. Results of previous studies are examined in detail, and commonly occurring temperature stress response proteins are collated. We conclude that technical advances, and improvements in genome sequence availability, will have an ever increasing impact on our understanding of molecular mechanisms of stress response in plants.
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Keywords
Keywords: heat shock protein; proteome; vegetable protein; analytic method; Arabidopsis; aspen; barley; cold stress; down regulation; gene expression regulation; genome analysis; heat stress; intermethod comparison; isotope labeling; nonhuman; pea; plant developmen 2-DE; Plant proteomics; Quantitative proteomics; Shotgun proteomics; Temperature stress
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Source
Proteomics
Type
Journal article
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Restricted until
2037-12-31
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