Gene editing of the multi-copy H2A.B gene and its importance for fertility 06 Biological Sciences 0604 Genetics Jin-Soo Kim
Date
2019
Authors
Anuar, Nur Diana
Kurscheid, Sebastian
Field, Matthew
Zhang, Lei
Rebar, Edward
Gregory, Philip
Buchou, Thierry
Bowles, Josephine
Koopman, Peter
Tremethick, David
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BioMed Central
Abstract
Altering the biochemical makeup of chromatin by the incorporation of histone variants during development represents a key mechanism in regulating gene expression. The histone variant H2A.B, H2A.B.3 in mice, appeared late in evolution and is most highly expressed in the testis. In the mouse, it is encoded by three different genes. H2A.B expression is spatially and temporally regulated during spermatogenesis being most highly expressed in the haploid round spermatid stage. Active genes gain H2A.B where it directly interacts with polymerase II and RNA processing factors within splicing speckles. However, the importance of H2A.B for gene expression and fertility are unknown.
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Keywords
Chromatin, Histone variants, H2A.B, RNA polymerase II, Pre-mRNA splicing, Splicing speckles, Genome editing, TALENs
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Source
Genome Biology
Type
Journal article
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Access Statement
Open Access
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Creative Commons Attribution licence
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