Rockendorf, Niels; Bade, Steffen; Hirst, Timothy; Gorris, Hans H.; Frey, Andreas
A ganglioside GM1 probe bearing a dark-red fluorescent dye at the sphingosine moiety of the molecule was prepared by a convenient one-pot synthesis. The labeled GM1 permitted the detection of the natural ganglioside GM1 ligand Escherichia coli heat-labile enterotoxin subunit B (EtxB) in picomole quantities on a solid support. When an epitope mapping of several ganglioside binding proteins and protein fragments was performed by screening a cellulose membrane-bound synthetic library of 64 16mer...[Show more]
Items in Open Research are protected by copyright, with all rights reserved, unless otherwise indicated.