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Synthesis of a Fluorescent Ganglioside G M1 Derivative and Sscreening of a Synthetic Peptide Library for G M1 Binding Sequence Motifs

Rockendorf, Niels; Bade, Steffen; Hirst, Timothy; Gorris, Hans H.; Frey, Andreas


A ganglioside GM1 probe bearing a dark-red fluorescent dye at the sphingosine moiety of the molecule was prepared by a convenient one-pot synthesis. The labeled GM1 permitted the detection of the natural ganglioside GM1 ligand Escherichia coli heat-labile enterotoxin subunit B (EtxB) in picomole quantities on a solid support. When an epitope mapping of several ganglioside binding proteins and protein fragments was performed by screening a cellulose membrane-bound synthetic library of 64 16mer...[Show more]

CollectionsANU Research Publications
Date published: 2007
Type: Journal article
Source: Bioconjugate Chemistry
DOI: 10.1021/bc0602376


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