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Structural and genetic characterization of the Shigella boydii type 10 and type 6 O antigens

Senchenkova, Sof'ya N; Feng, Lu; Yang, Jinghua; Shashkov, Alexander S; Cheng, Jiansong; Liu, Dan; Knirel, Yuriy; Reeves, Peter R; Jin, Qi; Ye, Qiang; Wang, Lei

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Comparison of the O antigens of Shigella boydii types 10 and 6 by chemical analysis and nuclear magnetic resonance spectroscopy showed that their structures are similar, with the only difference being the presence or absence of D-ribofuranose, which is the immunodominant sugar in S. boydii type 10. In S. boydii type 6, a residue previously reported as α-D-GlcpA, was shown to be β-D-GlcpA as in S. boydii type 10. S. boydii types 10 and 6 are reported not to cross-react serologically, and the...[Show more]

dc.contributor.authorSenchenkova, Sof'ya N
dc.contributor.authorFeng, Lu
dc.contributor.authorYang, Jinghua
dc.contributor.authorShashkov, Alexander S
dc.contributor.authorCheng, Jiansong
dc.contributor.authorLiu, Dan
dc.contributor.authorKnirel, Yuriy
dc.contributor.authorReeves, Peter R
dc.contributor.authorJin, Qi
dc.contributor.authorYe, Qiang
dc.contributor.authorWang, Lei
dc.date.accessioned2015-12-07T22:46:45Z
dc.identifier.issn0021-9193
dc.identifier.urihttp://hdl.handle.net/1885/25906
dc.description.abstractComparison of the O antigens of Shigella boydii types 10 and 6 by chemical analysis and nuclear magnetic resonance spectroscopy showed that their structures are similar, with the only difference being the presence or absence of D-ribofuranose, which is the immunodominant sugar in S. boydii type 10. In S. boydii type 6, a residue previously reported as α-D-GlcpA, was shown to be β-D-GlcpA as in S. boydii type 10. S. boydii types 10 and 6 are reported not to cross-react serologically, and the role of D-ribofuranose in the specificity of S. boydii was confirmed by making a mutant of type 10 that lacked D-ribofuranose. However, S. boydii type 11, which has a D-ribofuranose but with different linkage does show cross-reaction with type 10. The O-antigen gene loci of S. boydii types 10 and 6 were shown to be virtually identical except that orf8 (wbaM), which was confirmed as the ribofuranosyltransferase gene, is interrupted by IS629 in type 6. Therefore, it is proposed that the O-antigen gene cluster of S. boydii type 6 was derived from type 10 by an IS element insertion.
dc.publisherAmerican Society for Microbiology
dc.sourceJournal of Bacteriology
dc.subjectKeywords: dextro ribofuranose; O antigen; ribofuranosyltransferase; sugar; transferase; unclassified drug; article; chemical analysis; cross reaction; gene cluster; gene identification; gene insertion; gene locus; gene structure; immunogenicity; linkage analysis; m
dc.titleStructural and genetic characterization of the Shigella boydii type 10 and type 6 O antigens
dc.typeJournal article
local.description.notesImported from ARIES
local.identifier.citationvolume187
dc.date.issued2005
local.identifier.absfor030400 - MEDICINAL AND BIOMOLECULAR CHEMISTRY
local.identifier.ariespublicationu4222028xPUB41
local.type.statusPublished Version
local.contributor.affiliationSenchenkova, Sof'ya N, Russian Academy of Sciences
local.contributor.affiliationFeng, Lu, Nankai University
local.contributor.affiliationYang, Jinghua, Nankai University
local.contributor.affiliationShashkov, Alexander S, Russian Academy of Sciences
local.contributor.affiliationCheng, Jiansong, Nankai University
local.contributor.affiliationLiu, Dan, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationKnirel, Yuriy, Russian Academy of Sciences
local.contributor.affiliationReeves, Peter R, University of New South Wales
local.contributor.affiliationJin, Qi, State Key Laboratory for Molecular Virology
local.contributor.affiliationYe, Qiang, National Institute for the Control of Pharmaceutical and Biological Products
local.contributor.affiliationWang, Lei, Nankai University
local.description.embargo2037-12-31
local.bibliographicCitation.issue7
local.bibliographicCitation.startpage2551
local.bibliographicCitation.lastpage2554
local.identifier.doi10.1128/JB.187.7.2551-2554.2005
dc.date.updated2015-12-07T11:45:15Z
local.identifier.scopusID2-s2.0-20144366308
CollectionsANU Research Publications

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