Helicobacter pylori induces somatic mutations in TP53 via overexpression of CHAC1 in infected gastric epithelial cells
Date
2018
Authors
Wada, Yuriko
Takemura, Kosuke
Tummala, Padmaja
Uchida, Keisuke
Kitagaki, Keisuke
Furukawa, Asuka
Ishige, Yuuki
Ito, Takashi
Hara, Yukichi
Suzuki, Takashige
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Volume Title
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Wiley Blackwell
Abstract
Infection with Helicobacter pylori is known to decrease the level of glutathione in gastric epithelial cells and increase the production of reactive oxygen species (ROS), which can lead to DNA damage and the development of gastric cancer. Cation transport regulator 1 (CHAC1) has γ-glutamylcyclotransferase activity that degrades glutathione. We found that cagA-positive H. pylori infection triggered CHAC1 overexpression in human gastric epithelial (AGS) cells leading to glutathione degradation and the accumulation of ROS. Nucleotide alterations in the TP53 tumour suppressor gene were induced in AGS cells overexpressing CHAC1, whereas no mutations were detected in cells overexpressing a catalytically inactive mutant of CHAC1. A high frequency of TP53 mutations occurred in H. pylori-infected AGS cells, but this was prevented in cells transfected with CHAC1 siRNA. These findings indicate that H. pylori-mediated CHAC1 overexpression degrades intracellular glutathione, allowing the accumulation of ROS which subsequently causes mutations that could contribute to the development of gastric cancer.
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Keywords
cagA, CHAC1, glutathione, H. pylori, p53, ROS
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FEBS Open Bio
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Journal article
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Open Access
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Creative Commons License (Attribution 4.0 International)
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