Mannitol is required for asexual sporulation in the wheat pathogen Stagonospora nodorum (glume blotch)
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Solomon, Peter; Waters, Ormonde D. C.; Jörgens, Cordula I.; Lowe, Rohan G T; Rechberger, Judith; Trengrove, Robert D.; Oliver, Richard Peter
Description
The physiological role of the mannitol cycle in the wheat pathogen Stagonospora nodorum (glume blotch) has been investigated by reverse genetics and metabolite profiling. A putative mannitol 2-dehydrogenase gene (Mdh1) was cloned by degenerate PCR and disrupted. The resulting mutated mdh1 strains lacked all detectable NADPH-dependent mannitol dehydrogenase activity. The mdh1 strains were unaffected for mannitol production but, surprisingly, were still able to utilize mannitol as a sole carbon...[Show more]
Collections | ANU Research Publications |
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Date published: | 2006 |
Type: | Journal article |
URI: | http://hdl.handle.net/1885/23547 |
Source: | Biochemical Journal |
DOI: | 10.1042/BJ20060891 |
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01_Solomon_Mannitol_is_required_for_2006.pdf | 328.86 kB | Adobe PDF |
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