Skip navigation
Skip navigation

Mannitol is required for asexual sporulation in the wheat pathogen Stagonospora nodorum (glume blotch)

Solomon, Peter; Waters, Ormonde D. C.; Jörgens, Cordula I.; Lowe, Rohan G T; Rechberger, Judith; Trengrove, Robert D.; Oliver, Richard Peter

Description

The physiological role of the mannitol cycle in the wheat pathogen Stagonospora nodorum (glume blotch) has been investigated by reverse genetics and metabolite profiling. A putative mannitol 2-dehydrogenase gene (Mdh1) was cloned by degenerate PCR and disrupted. The resulting mutated mdh1 strains lacked all detectable NADPH-dependent mannitol dehydrogenase activity. The mdh1 strains were unaffected for mannitol production but, surprisingly, were still able to utilize mannitol as a sole carbon...[Show more]

dc.contributor.authorSolomon, Peter
dc.contributor.authorWaters, Ormonde D. C.
dc.contributor.authorJörgens, Cordula I.
dc.contributor.authorLowe, Rohan G T
dc.contributor.authorRechberger, Judith
dc.contributor.authorTrengrove, Robert D.
dc.contributor.authorOliver, Richard Peter
dc.date.accessioned2015-12-07T22:38:43Z
dc.identifier.issn0264-6021
dc.identifier.urihttp://hdl.handle.net/1885/23547
dc.description.abstractThe physiological role of the mannitol cycle in the wheat pathogen Stagonospora nodorum (glume blotch) has been investigated by reverse genetics and metabolite profiling. A putative mannitol 2-dehydrogenase gene (Mdh1) was cloned by degenerate PCR and disrupted. The resulting mutated mdh1 strains lacked all detectable NADPH-dependent mannitol dehydrogenase activity. The mdh1 strains were unaffected for mannitol production but, surprisingly, were still able to utilize mannitol as a sole carbon source, suggesting a hitherto unknown mechanism for mannitol catabolism. The mutant strains were not compromised in their ability to cause disease or sporulate. To further our understanding of mannitol metabolism, a previously developed mannitol-1-phosphate dehydrogenase (gene mpd1) disruption construct [Solomon, Tan and Oliver (2005) Mol. Plant-Microbe Interact. 18, 110-115] was introduced into the mutated mdh1 background, resulting in a strain lacking both enzyme activities. The mpd1 mdh1 strains were unable to grow on mannitol and produced only trace levels of mannitol. The double-mutant strains were unable to sporulate in vitro when grown on minimal medium for extended periods. Deficiency in sporulation was correlated with the depletion of intracellular mannitol pools. Significantly sporulation could be restored with the addition of mannitol. Pathogenicity of the double mutant was not compromised, although, like the previously characterized mpd1 mutants, the strains were unable to sporulate in planta. These findings not only question the currently hypothesized pathways of mannitol metabolism, but also identify for the first time that mannitol is required for sporulation of a filamentous fungus.
dc.publisherPortland Press
dc.sourceBiochemical Journal
dc.subjectKeywords: Metabolomics; Primary fungal metabolism; Sporulation; Stagonospora nodorum (glume blotch); Wheat fungal pathogen; Bacteria; Genes; Metabolites; Pathology; Crops; mannitol; mannitol 1 phosphate dehydrogenase enzyme; mannitol dehydrogenase; mannitol phospha Mannitol; Metabolomics; Primary fungal metabolism; Sporulation; Stagonospora nodorum (glume blotch); Wheat fungal pathogen
dc.titleMannitol is required for asexual sporulation in the wheat pathogen Stagonospora nodorum (glume blotch)
dc.typeJournal article
local.description.notesImported from ARIES
local.identifier.citationvolume399
dc.date.issued2006
local.identifier.absfor060700 - PLANT BIOLOGY
local.identifier.ariespublicationu4692404xPUB27
local.type.statusPublished Version
local.contributor.affiliationSolomon, Peter, College of Medicine, Biology and Environment, ANU
local.contributor.affiliationWaters, Ormonde D. C., Murdoch University
local.contributor.affiliationJörgens, Cordula I., Murdoch University
local.contributor.affiliationLowe, Rohan G T, Murdoch University
local.contributor.affiliationRechberger, Judith, Murdoch University
local.contributor.affiliationTrengrove, Robert D., Murdoch University
local.contributor.affiliationOliver, Richard Peter, Murdoch University
local.description.embargo2037-12-31
local.bibliographicCitation.issue2
local.bibliographicCitation.startpage231
local.bibliographicCitation.lastpage239
local.identifier.doi10.1042/BJ20060891
dc.date.updated2015-12-07T10:40:31Z
local.identifier.scopusID2-s2.0-33750029714
CollectionsANU Research Publications

Download

File Description SizeFormat Image
01_Solomon_Mannitol_is_required_for_2006.pdf328.86 kBAdobe PDF    Request a copy


Items in Open Research are protected by copyright, with all rights reserved, unless otherwise indicated.

Updated:  19 May 2020/ Responsible Officer:  University Librarian/ Page Contact:  Library Systems & Web Coordinator