Enzyme Encapsulation in a Porous Hydrogen-Bonded Organic Framework
Date
2019
Authors
Liang, Weibin
Carraro, Francesco
Solomon, Marcello B.
Bell, Stephen
Amenitsch, Heinz
Sumby, Christopher J
White, Nicholas
Falcaro, Paolo
Doonan, Christian J.
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Volume Title
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American Chemical Society
Abstract
Protection of biological assemblies is critical to
applications in biotechnology, increasing the durability of enzymes in
biocatalysis or potentially stabilizing biotherapeutics during transport
and use. Here we show that a porous hydrogen-bonded organic
framework (HOF) constructed from water-soluble tetra-amidinium (1ยท
Cl4) and tetracarboxylate (2) building blocks can encapsulate and
stabilize biomolecules to elevated temperature, proteolytic and
denaturing agents, and extend the operable pH range for catalase
activity. The HOF, which readily retains water within its framework
structure, can also protect and retain the activity of enzymes such as
alcohol oxidase, that are inactive when encapsulated within zeolitic
imidazolate framework (ZIF) materials. Such HOF coatings could
provide valid alternative materials to ZIFs: they are metal free, possess
larger pore apertures, and are stable over a wider, more biologically
relevant pH range.
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Journal of the American Chemical Society
Type
Journal article
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2037-12-31
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