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MTrack: Automated Detection, Tracking, and Analysis of Dynamic Microtubules

Kapoor, Varun; Hirst, William; Hentschel, Christoph; Preibisch, Stephan; Reber, Simone

Description

Microtubules are polar, dynamic filaments fundamental to many cellular processes. In vitro reconstitution approaches with purified tubulin are essential to elucidate different aspects of microtubule behavior. To date, deriving data from fluorescence microscopy images by manually creating and analyzing kymographs is still commonplace. Here, we present MTrack, implemented as a plug-in for the open-source platform Fiji, which automatically identifies and tracks dynamic microtubules with sub-pixel...[Show more]

CollectionsANU Research Publications
Date published: 2019
Type: Journal article
URI: http://hdl.handle.net/1885/201739
Source: Scientific Reports
DOI: 10.1038/s41598-018-37767-1
Access Rights: Open Access

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