Wu, Nan; Zhu, Yonghau; Brown, S; Oakeshott, John Graham; Peat, T.S.; Surjadi, R; Easton, Christopher; Leech, P W; Sexton, B A
Droplet based microfluidics are promising new tools for biological and chemical assays. In this paper, a high throughput and high sensitivity microfluidic droplet platform is described for in vitro protein expression using crude Escherichia coli S30 extract. A flow-focusing polymethylmethacrylate (PMMA) microchip was designed and integrated with different functions involving droplet generation, storage, separation and detection. The material used for the chip is superior to the previously...[Show more]
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