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Glutathione export from human erythrocytes and Plasmodium falciparum malaria parasites

Barrand, Margery A.; Winterberg, Markus; Ng, Frances; Mai, Nguyen; Kirk, Kiaran; Hladky, Stephen B.


Glutathione export from uninfected human erythrocytes was compared with that from cells infected with the malaria parasite Plasmodium falciparum using two separate methods that distinguish between oxidized (GSSG) and reduced (GSH) glutathione. One involved enzymatic recycling with or without thiol-masking; the other involved rapid derivatization followed by HPLC. Glutathione efflux from uninfected erythrocytes under physiological conditions occurred predominantly as GSH. On exposure of the...[Show more]

CollectionsANU Research Publications
Date published: 2012
Type: Journal article
Source: Biochemical Journal
DOI: 10.1042/BJ20121050


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