Small Gd(III) Tags for Gd(III)-Gd(III) Distance Measurements in Proteins by EPR Spectroscopy
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Prokopiou, Georgia; Lee, Michael D.; Collauto, A.; Abdelkader, Elwy; Bahrenberg, Thorsten; Feintuch, Akiva; Ramirez Cohen, Marie; Clayton, Jessica; Swarbrick, James; Graham, Bim; Otting, Gottfried; Goldfarb, Daniella
Description
The C7-Gd and C8-Gd tags are compact hydrophilic cyclen-based lanthanide tags for conjugation to cysteine residues in proteins. The tags are enantiomers, which differ in the configuration of the 2-hydroxylpropyl pendant arms coordinating the lanthanide ion. Here, we report the electron paramagnetic resonance (EPR) performance of the C7-Gd (S configuration) and C8-Gd (R configuration) tags loaded with Gd(III) on two mutants of the homodimeric ERp29 protein. The W-band EPR spectra were found to...[Show more]
Collections | ANU Research Publications |
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Date published: | 2018 |
Type: | Journal article |
URI: | http://hdl.handle.net/1885/164995 |
Source: | Inorganic Chemistry |
DOI: | 10.1021/acs.inorgchem.8b00133 |
Access Rights: | Open Access |
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Small Gd(III) Tags for Gd(III)-Gd(III) Distance.pdf | 1.81 MB | Adobe PDF |
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