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Carboxysome encapsulation of the CO₂-fixing enzyme Rubisco in tobacco chloroplasts

Long, Benedict; Hee, Wei Yih; Sharwood, Robert; Rae, Benjamin; Kaines, Sarah; Lim, Yi Leen; Nguyen, Nghiem Dinh; Massey, Baxter; Bala, Soumi; von Caemmerer, Susanne; Badger, Murray; Price, Graeme (Dean)

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A long-term strategy to enhance global crop photosynthesis and yield involves the introduction of cyanobacterial CO2-concentrating mechanisms (CCMs) into plant chloroplasts. Cyanobacterial CCMs enable relatively rapid CO2 fixation by elevating intracellular inorganic carbon as bicarbonate, then concentrating it as CO2 around the enzyme Rubisco in specialized protein micro-compartments called carboxysomes. To date, chloroplastic expression of carboxysomes has been elusive, requiring coordinated...[Show more]

dc.contributor.authorLong, Benedict
dc.contributor.authorHee, Wei Yih
dc.contributor.authorSharwood, Robert
dc.contributor.authorRae, Benjamin
dc.contributor.authorKaines, Sarah
dc.contributor.authorLim, Yi Leen
dc.contributor.authorNguyen, Nghiem Dinh
dc.contributor.authorMassey, Baxter
dc.contributor.authorBala, Soumi
dc.contributor.authorvon Caemmerer, Susanne
dc.contributor.authorBadger, Murray
dc.contributor.authorPrice, Graeme (Dean)
dc.date.accessioned2019-08-08T03:04:53Z
dc.date.available2019-08-08T03:04:53Z
dc.identifier.issn2041-1723
dc.identifier.urihttp://hdl.handle.net/1885/164922
dc.description.abstractA long-term strategy to enhance global crop photosynthesis and yield involves the introduction of cyanobacterial CO2-concentrating mechanisms (CCMs) into plant chloroplasts. Cyanobacterial CCMs enable relatively rapid CO2 fixation by elevating intracellular inorganic carbon as bicarbonate, then concentrating it as CO2 around the enzyme Rubisco in specialized protein micro-compartments called carboxysomes. To date, chloroplastic expression of carboxysomes has been elusive, requiring coordinated expression of almost a dozen proteins. Here we successfully produce simplified carboxysomes, isometric with those of the source organism Cyanobium, within tobacco chloroplasts. We replace the endogenous Rubisco large subunit gene with cyanobacterial Form-1A Rubisco large and small subunit genes, along with genes for two key α-carboxysome structural proteins. This minimal gene set produces carboxysomes, which encapsulate the introduced Rubisco and enable autotrophic growth at elevated CO2. This result demonstrates the formation of α-carboxysomes from a reduced gene set, informing the step-wise construction of fully functional α-carboxysomes in chloroplasts.
dc.format.mimetypeapplication/pdf
dc.language.isoen_AU
dc.publisherNature Research
dc.rights© The Author(s) 2018
dc.rights.urihttp://creativecommons.org/ licenses/by/4.0/
dc.sourceNature Communications
dc.titleCarboxysome encapsulation of the CO₂-fixing enzyme Rubisco in tobacco chloroplasts
dc.typeJournal article
local.description.notesImported from ARIES
local.identifier.citationvolume9
dc.date.issued2018
local.identifier.absfor060705 - Plant Physiology
local.identifier.absfor060702 - Plant Cell and Molecular Biology
local.identifier.ariespublicationu5786633xPUB424
local.publisher.urlhttps://www.nature.com/
local.type.statusPublished Version
local.contributor.affiliationLong, Benedict, College of Science, ANU
local.contributor.affiliationHee, Wei, College of Science, ANU
local.contributor.affiliationSharwood, Robert, College of Science, ANU
local.contributor.affiliationRae, Benjamin, College of Science, ANU
local.contributor.affiliationKaines, Sarah, College of Science, ANU
local.contributor.affiliationLim, Yi-Leen, College of Science, ANU
local.contributor.affiliationNguyen, Nghiem, College of Science, ANU
local.contributor.affiliationMassey, Baxter, College of Science, ANU
local.contributor.affiliationBala, Soumi, College of Science, ANU
local.contributor.affiliationvon Caemmerer, Susanne, College of Science, ANU
local.contributor.affiliationBadger, Murray, College of Science, ANU
local.contributor.affiliationPrice, Graeme (Dean), College of Science, ANU
dc.relationhttp://purl.org/au-research/grants/arc/CE140100015
local.bibliographicCitation.issue1
local.bibliographicCitation.startpage1
local.bibliographicCitation.lastpage14
local.identifier.doi10.1038/s41467-018-06044-0
local.identifier.absseo970106 - Expanding Knowledge in the Biological Sciences
dc.date.updated2019-03-31T07:24:37Z
local.identifier.scopusID2-s2.0-85052760896
dcterms.accessRightsOpen Access
dc.provenanceThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/ licenses/by/4.0/.
dc.rights.licenseCreative Commons Attribution 4.0 International License
CollectionsANU Research Publications

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