Mitochondrial DNA and trade data support multiple origins of Helicoverpa armigera (Lepidoptera, Noctuidae) in Brazil
Date
2018
Authors
Tay, Wee Tek
Walsh, T.K.
Downes, Sharon
Anderson, Craig
Jermiin, Lars
Wong, Thomas
Piper, Melissa
Chang, Ester Silva
Macedo, Isabella Barony
Czepak, Cecilia
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Nature Research
Abstract
The Old World bollworm Helicoverpa armigera
is now established in Brazil but efforts to identify
incursion origin(s) and pathway(s) have met with limited success due to the patchiness of available data. Using international agricultural/horticultural commodity trade data and mitochondrial DNA (mtDNA)
cytochrome oxidase I (COI) and cytochrome b (Cyt
b) gene markers, we inferred the origins and incursion
pathways into Brazil. We detected 20 mtDNA haplotypes from six Brazilian states, eight of which were new to our 97 global COI-Cyt b haplotype database. Direct sequence matches indicated five Brazilian haplotypes had Asian, African, and European origins. We identified 45 parsimoniously informative
sites and multiple substitutions per site within the concatenated (945 bp) nucleotide dataset, implying that probabilistic phylogenetic analysis methods are needed. High diversity and signatures of uniquely shared haplotypes with diverse localities combined with the trade data suggested multiple incursions
and introduction origins in Brazil. Increasing agricultural/horticultural trade activities between the
Old and New Worlds represents a significant biosecurity risk factor. Identifying pest origins will enable resistance profiling that reflects countries of origin to be included when developing a resistance
management strategy, while identifying incursion pathways will improve biosecurity protocols and risk
analysis at biosecurity hotspots including national ports
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Nature
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Journal article
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Open Access
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Creative Commons Attribution 4.0 International License
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