Directed -in vitro- evolution of Precambrian and extant Rubiscos
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Gomez-Fernandez, Bernardo; Garcia-Ruiz, Eva; Martin-Diaz, Javier; de Santos, Patricia Gomez; Santos-Moriano, Paloma; Plou, Francisco J.; Ballesteros, Antonio; Garcia, Monica; Rodriguez, Marisa; Risso, Valeria A.; Sanchez-Ruiz, Jose M.; Whitney, Spencer
Description
Rubisco is an ancient, catalytically conserved yet slow enzyme, which plays a central role in the biosphere’s carbon cycle. The design of Rubiscos to increase agricultural productivity has hitherto relied on the use of in vivo selection systems, precluding the exploration of biochemical traits that are not wired to cell survival. We present a directed -in vitro- evolution platform that extracts the enzyme from its biological context to provide a new avenue for Rubisco engineering. Precambrian...[Show more]
dc.contributor.author | Gomez-Fernandez, Bernardo | |
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dc.contributor.author | Garcia-Ruiz, Eva | |
dc.contributor.author | Martin-Diaz, Javier | |
dc.contributor.author | de Santos, Patricia Gomez | |
dc.contributor.author | Santos-Moriano, Paloma | |
dc.contributor.author | Plou, Francisco J. | |
dc.contributor.author | Ballesteros, Antonio | |
dc.contributor.author | Garcia, Monica | |
dc.contributor.author | Rodriguez, Marisa | |
dc.contributor.author | Risso, Valeria A. | |
dc.contributor.author | Sanchez-Ruiz, Jose M. | |
dc.contributor.author | Whitney, Spencer | |
dc.date.accessioned | 2019-04-21T04:38:46Z | |
dc.date.available | 2019-04-21T04:38:46Z | |
dc.identifier.issn | 2045-2322 | |
dc.identifier.uri | http://hdl.handle.net/1885/160533 | |
dc.description.abstract | Rubisco is an ancient, catalytically conserved yet slow enzyme, which plays a central role in the biosphere’s carbon cycle. The design of Rubiscos to increase agricultural productivity has hitherto relied on the use of in vivo selection systems, precluding the exploration of biochemical traits that are not wired to cell survival. We present a directed -in vitro- evolution platform that extracts the enzyme from its biological context to provide a new avenue for Rubisco engineering. Precambrian and extant form II Rubiscos were subjected to an ensemble of directed evolution strategies aimed at improving thermostability. The most recent ancestor of proteobacteria -dating back 2.4 billion years- was uniquely tolerant to mutagenic loading. Adaptive evolution, focused evolution and genetic drift revealed a panel of thermostable mutants, some deviating from the characteristic trade-offs in CO2-fixing speed and specificity. Our findings provide a novel approach for identifying Rubisco variants with improved catalytic evolution potential. | |
dc.format.mimetype | application/pdf | |
dc.language.iso | en_AU | |
dc.publisher | Nature Publishing Group | |
dc.rights | The Authors | |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.source | Scientific Reports | |
dc.title | Directed -in vitro- evolution of Precambrian and extant Rubiscos | |
dc.type | Journal article | |
local.description.notes | Imported from ARIES | |
local.identifier.citationvolume | 8 | |
dc.date.issued | 2018 | |
local.identifier.absfor | 060705 - Plant Physiology | |
local.identifier.ariespublication | u4485658xPUB2094 | |
local.type.status | Published Version | |
local.contributor.affiliation | Gomez-Fernandez, Bernardo, Institute of Catalysis, CSIC | |
local.contributor.affiliation | Garcia-Ruiz, Eva, Institute of Catalysis, CSIC | |
local.contributor.affiliation | Martin-Diaz, Javier, Institute of Catalysis, CSIC | |
local.contributor.affiliation | de Santos, Patricia Gomez, Institute of Catalysis, CSIC | |
local.contributor.affiliation | Santos-Moriano, Paloma, Institute of Catalysis, CSIC | |
local.contributor.affiliation | Plou, Francisco J., Institute of Catalysis, CSIC | |
local.contributor.affiliation | Ballesteros, Antonio, Institute of Catalysis, CSIC | |
local.contributor.affiliation | Garcia, Monica, Centro del Tecnología Química | |
local.contributor.affiliation | Rodriguez, Marisa, Centro del Tecnología Química | |
local.contributor.affiliation | Risso, Valeria A., Universidad de Granada | |
local.contributor.affiliation | Sanchez-Ruiz, Jose M., Universidad de Granada | |
local.contributor.affiliation | Whitney, Spencer, College of Science, ANU | |
local.bibliographicCitation.issue | 5532 | |
local.identifier.doi | 10.1038/s41598-018-23869-3 | |
local.identifier.absseo | 970106 - Expanding Knowledge in the Biological Sciences | |
dc.date.updated | 2019-03-12T07:32:42Z | |
local.identifier.scopusID | 2-s2.0-85044972767 | |
local.identifier.thomsonID | 000428999200068 | |
dcterms.accessRights | Open Access | |
dc.rights.license | This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Cre- ative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not per- mitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. | |
Collections | ANU Research Publications |
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