Optical toolkits for in vivo deep tissue laser scanning microscopy: a primer
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Lee, Woei Ming (Steve); McMenamin, Thomas; Li, Yongxiao
Description
Life at the microscale is animated and multifaceted. The impact of dynamic in vivo microscopy in small animals has opened up opportunities to peer into a multitude of biological processes at the cellular scale in their native microenvironments. Laser scanning microscopy (LSM) coupled with targeted fluorescent proteins has become an indispensable tool to enable dynamic imaging in vivo at high temporal and spatial resolutions. In the last few decades, the technique has been translated from...[Show more]
dc.contributor.author | Lee, Woei Ming (Steve) | |
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dc.contributor.author | McMenamin, Thomas | |
dc.contributor.author | Li, Yongxiao | |
dc.date.accessioned | 2019-04-10T11:08:25Z | |
dc.identifier.issn | 0150-536X | |
dc.identifier.uri | http://hdl.handle.net/1885/159451 | |
dc.description.abstract | Life at the microscale is animated and multifaceted. The impact of dynamic in vivo microscopy in small animals has opened up opportunities to peer into a multitude of biological processes at the cellular scale in their native microenvironments. Laser scanning microscopy (LSM) coupled with targeted fluorescent proteins has become an indispensable tool to enable dynamic imaging in vivo at high temporal and spatial resolutions. In the last few decades, the technique has been translated from imaging cells in thin samples to mapping cells in the thick biological tissue of living organisms. Here, we sought to provide a concise overview of the design considerations of a LSM that enables cellular and subcellular imaging in deep tissue. Individual components under review include: long working distance microscope objectives, laser scanning technologies, adaptive optics devices, beam shaping technologies and photon detectors, with an emphasis on more recent advances. The review will conclude with the latest innovations in automated optical microscopy, which would impact tracking and quantification of heterogeneous populations of cells in vivo. | |
dc.format.mimetype | application/pdf | |
dc.language.iso | en_AU | |
dc.publisher | Institute of Physics Publishing | |
dc.source | Journal of Optics | |
dc.title | Optical toolkits for in vivo deep tissue laser scanning microscopy: a primer | |
dc.type | Journal article | |
local.description.notes | Imported from ARIES | |
local.identifier.citationvolume | 20 | |
dc.date.issued | 2018 | |
local.identifier.absfor | 090606 - Photonics and Electro-Optical Engineering (excl. Communications) | |
local.identifier.absfor | 060106 - Cellular Interactions (incl. Adhesion, Matrix, Cell Wall) | |
local.identifier.ariespublication | u1029610xPUB127 | |
local.type.status | Published Version | |
local.contributor.affiliation | Lee, Woei Ming (Steve), College of Engineering and Computer Science, ANU | |
local.contributor.affiliation | McMenamin, Thomas, College of Engineering and Computer Science, ANU | |
local.contributor.affiliation | Li, Yongxiao, College of Engineering and Computer Science, ANU | |
local.description.embargo | 2039-12-31 | |
local.bibliographicCitation.issue | 6 | |
local.identifier.doi | 10.1088/2040-8986/aac4af2040-8978/ | |
local.identifier.absseo | 861500 - INSTRUMENTATION | |
dc.date.updated | 2019-03-12T07:23:48Z | |
local.identifier.scopusID | 2-s2.0-85047857495 | |
dc.provenance | Journal: Journal of Optics (ISSN: 2040-8978, ESSN: 2040-8986) [Formerly known as Journal of Optics A: Pure and Applied Optics] RoMEO: This is a RoMEO green journal Paid OA: A paid open access option is available for this journal. Author's Pre-print: green tick author can archive pre-print (ie pre-refereeing) Author's Post-print: green tick author can archive post-print (ie final draft post-refereeing) Publisher's Version/PDF: cross author cannot archive publisher's version/PDF | |
Collections | ANU Research Publications |
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