DHMI: Dynamic holographic microscopy interface

Date

2016

Authors

He, Xuefei
Zheng, Yujie
Lee, Woei Ming (Steve)

Journal Title

Journal ISSN

Volume Title

Publisher

SPIE - The International Society for Optical Engineering

Abstract

Digital holographic microscopy (DHM) is a powerful in-vitro biological imaging tool. In this paper, we report a fully automated off-axis digital holographic microscopy system completed with a graphical user interface in the Matlab © environment. The interface primarily includes Fourier domain processing, phase reconstruction, aberration compensation and autofocusing. A variety of imaging operations such as region of interest selection, de-noising mode (filtering and averaging), low frame rate imaging for immediate reconstruction and high frame rate imaging routine (~ 27 fps) are implemented to facilitate ease of use.

Description

Keywords

Citation

Source

Proceedings of SPIE, Conference Volume 10013

Type

Conference paper

Book Title

Entity type

Access Statement

Open Access

License Rights

Restricted until