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Generation of pseudocontact shifts in proteins with lanthanides using small “clickable” nitrilotriacetic acid and iminodiacetic acid tags

Loh, Choy-Theng; Graham, Bim; Abdelkader, Elwy H.; Tuck, Kellie L.; Otting, Gottfried


Pseudocontact shifts (PCS) induced by paramagnetic lanthanide ions provide unique long-range structural information in nuclear magnetic resonance (NMR) spectra, but the site-specific attachment of lanthanide tags to proteins remains a challenge. Here we incorporated p-azido-phenylalanine (AzF) site-specifically into the proteins ubiquitin and GB1, and ligated the AzF residue with alkyne derivatives of small nitrilotriacetic acid and iminodiacetic acid tags using the Cu(I) -catalysed "click"...[Show more]

CollectionsANU Research Publications
Date published: 2015
Type: Journal article
Source: Chemistry - A European Journal
DOI: 10.1002/chem.201406274


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