Genes expressed in zoospores of Phytophthora nicotianae
Date
2004-01
Authors
Śkalamera, D
Wasson, A P
Hardham, Adrienne R
Journal Title
Journal ISSN
Volume Title
Publisher
Springer Verlag
Abstract
The genus Phytophthora includes many highly
destructive plant pathogens. In many
Phytophthora species, pathogen dispersal and initiation of plant infection are achieved by motile, biflagellate zoospores that are
chemotactically attracted to suitable infection sites. In order to study gene expression in zoospores, we have constructed a cDNA library using mRNA from zoospores of
Phytophthora nicotianae. The library was arrayed and screened using probes derived from mycelium or zoospore mRNA. More than 400 clones representing genes preferentially expressed in zoospores were identified and sequenced from the 5' end of the insert. The
expressed sequence tags (ESTs) generated were found to represent 240 genes. The ESTs were compared to sequences in GenBank and in the Phytophthora Genome Consortium database, and classified according to putative function based on homology to known proteins. To further characterize the identified genes, a colony array was created on replicate nylon filters and screened with
probes derived from four Phytophthora
developmental stages including zoospores, germinating cysts, vegetative mycelium and sporulating hyphae, and from inoculated
and uninoculated tobacco seedlings. Data from sequence analysis and colony array screening were compiled into a local database, and searched to identify genes that are preferentially expressed in zoospores for future functional analysis.
Description
Keywords
gene, expression, oomycetes, plant, pathogen, interactions, Phytophthora, zoospores
Citation
Collections
Source
Molecular Genetics and Genomics 270. 6 (2004): 549–557
Type
Journal article