Truncation of gene F5L partially masks rescue of vaccinia virus strain MVA growth on mammalian cells by restricting plaque size
Modified vaccinia virus Ankara (MVA) is a candidate vaccine vector that is severely attenuated due to mutations acquired during several hundred rounds of serial passage in vitro. A previous study used marker rescue to produce a set of MVA recombinants with improved replication on mammalian cells. Here, we extended the characterization of these rescued MVA strains and identified vaccinia virus (VACV) gene F5L as a determinant of plaque morphology but not replication in vitro. F5 joins a growing...[Show more]
|Collections||ANU Research Publications|
|Source:||Journal of General Virology 95(Pt 2) (2014):466-71|
|Dobson and Tscharke Truncation of gene 2014.pdf||1.4 MB||Adobe PDF|
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