Extended Law of Corresponding States Applied to Solvent Isotope Effect on a Globular Protein
Date
2016-05-05
Authors
Bucciarelli, Saskia
Mahmoudi, Najet
Casal-Dujat, Lucía
Jéhannin, Marie
Jud, Corinne
Stradner, Anna
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American Chemical Society
Abstract
Investigating proteins with techniques such as NMR or neutron scattering frequently requires the partial or complete substitution of D₂O for H₂O as a solvent, often tacitly assuming that such a solvent substitution does not significantly alter the properties of the protein. Here, we report a systematic investigation of the solvent isotope effect on the phase diagram of the lens protein γB-crystallin in aqueous solution as a model system exhibiting liquid-liquid phase separation. We demonstrate that the observed strong variation of the critical temperature Tc can be described by the extended law of corresponding states for all H₂O/D₂O ratios, where scaling of the temperature by Tc or the reduced second virial coefficient accurately reproduces the binodal, spinodal, and osmotic compressibility. These findings highlight the impact of H₂O/D₂O substitution on γB-crystallin properties and warrant further investigations into the universality of this phenomenon and its underlying mechanisms.
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The journal of physical chemistry letters
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Journal article
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Open Access
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