Extended Law of Corresponding States Applied to Solvent Isotope Effect on a Globular Protein
Investigating proteins with techniques such as NMR or neutron scattering frequently requires the partial or complete substitution of D₂O for H₂O as a solvent, often tacitly assuming that such a solvent substitution does not significantly alter the properties of the protein. Here, we report a systematic investigation of the solvent isotope effect on the phase diagram of the lens protein γB-crystallin in aqueous solution as a model system exhibiting liquid-liquid phase separation. We demonstrate...[Show more]
|Collections||ANU Research Publications|
|Source:||The journal of physical chemistry letters|
|01_Bucciarelli_Extended_Law_2016.pdf||1.22 MB||Adobe PDF|
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