Technical advance: in vitro production of distinct dendritic-like antigen-presenting cells from self-renewing hematopoietic stem cells
Date
2011-11-10
Authors
Hinton, Rebecca A
O'Neill, Helen
Journal Title
Journal ISSN
Volume Title
Publisher
Society for Leukocyte Biology
Federation of American Societies for Experimental Biology (FASEB)
Federation of American Societies for Experimental Biology (FASEB)
Abstract
A novel CD11c(lo)CD11b(hi)MHC-II–CD8α– dendritic-like cell (L-DC) develops in cocultures of bone marrow over splenic stroma. L-DCs are distinct from other DC subsets and have potential importance in spleen for immunity to blood-borne antigens. As production is maintained in cultures for >12 months, L-DC development evidently depends on self-renewing progenitors. To improve this culture system, highly purified HSCs were sorted from bone marrow and used to establish cocultures. Nonadherent cells produced were analyzed for surface marker expression and capacity to activate/inhibit T cells. Cocultures produced a pure population of L-DCs for up to 12 months, which were strong activators of CD8+ T cells. The in vitro production of a pure population of L-DCs from HSCs—in numbers amenable to in vitro assays of function and development—therefore represents an important advance
Description
Keywords
Keywords: antigen presenting cell; article; bone marrow; CD8+ T lymphocyte; cell culture; cell function; cell population; cell regeneration; coculture; controlled study; hematopoietic stem cell; human; human cell; in vitro study; priority journal; protein expressio Hematopoiesis; Multiparameter FACS; Progenitors
Citation
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Source
Journal of Leukocyte Biology 91.2 (2012): 341-6
Type
Journal article