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The effect of surface charge on the thermal stability and ice recrystallization inhibition activity of antifreeze protein III (AFP III)

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dc.contributor.authorDeller, R. C.en
dc.contributor.authorCarter, B. M.en
dc.contributor.authorZampetakis, I.en
dc.contributor.authorScarpa, F.en
dc.contributor.authorPerriman, A. W.en
dc.date.accessioned2026-03-27T17:40:39Z
dc.date.available2026-03-27T17:40:39Z
dc.date.issued2018-01-01en
dc.description.abstractThe aim of this study was to examine the effect of chemical cationization on the structure and function of antifreeze protein III (AFP III) over an extreme temperature range (−40°C to +90°C) using far-UV synchrotron radiation circular dichroism (SRCD) and ice recrystallization inhibition (IRI) assays. Chemical cationization was able to produce a modified AFP III with a net cationic charge at physiological pH that had enhanced resistance to denaturation at elevated temperatures, with no immediate negative impact on protein structure at subzero temperatures. Furthermore, cationized AFP III retained an IRI activity similar to that of native AFP III. Consequently, chemical cationization may provide a pathway to the development of more robust antifreeze proteins as supplementary cryoprotectants in the cryopreservation of clinically relevant cells.en
dc.description.sponsorshipThis research was funded by the EPSRC ( EP/K026720/1 ). We thank Diamond Light Source for access to SRCD beamline B23 (SM12484) and the contributions of Dr. Giuliano Siligardi, Dr. Rohanah Hussain and Dr. Tamas Javorfi.en
dc.description.statusPeer-revieweden
dc.format.extent6en
dc.identifier.issn0006-291Xen
dc.identifier.otherPubMed:29137985en
dc.identifier.otherORCID:/0000-0003-2205-9364/work/209602497en
dc.identifier.scopus85034967155en
dc.identifier.urihttps://hdl.handle.net/1885/733807919
dc.language.isoenen
dc.rights©2017 The authorsen
dc.sourceBiochemical and Biophysical Research Communicationsen
dc.subjectAntifreeze proteinen
dc.subjectCryopreservationen
dc.subjectIce recrystallizationen
dc.subjectSynchrotron radiation circular dichroismen
dc.subjectThermal stabilityen
dc.titleThe effect of surface charge on the thermal stability and ice recrystallization inhibition activity of antifreeze protein III (AFP III)en
dc.typeJournal articleen
dspace.entity.typePublicationen
local.bibliographicCitation.lastpage1060en
local.bibliographicCitation.startpage1055en
local.contributor.affiliationDeller, R. C.; University of Bristolen
local.contributor.affiliationCarter, B. M.; University of Bristolen
local.contributor.affiliationZampetakis, I.; University of Bristolen
local.contributor.affiliationScarpa, F.; University of Bristolen
local.contributor.affiliationPerriman, A. W.; School of Cellular and Molecular Medicineen
local.identifier.citationvolume495en
local.identifier.doi10.1016/j.bbrc.2017.11.073en
local.identifier.pure1d3afbad-2675-4a34-b99e-ed1d7e8e41faen
local.identifier.urlhttps://www.scopus.com/pages/publications/85034967155en
local.type.statusPublisheden

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