Effect of Photobiomodulation on Proliferative Changes in the Retina: Evidence from an In Vitro Model of PVR
| dc.contributor.author | Watson, Oscar | en |
| dc.contributor.author | Aggio-Bruce, Riemke | en |
| dc.contributor.author | Essex, Rohan | en |
| dc.contributor.author | Valter, Krisztina | en |
| dc.date.accessioned | 2025-05-23T13:29:02Z | |
| dc.date.available | 2025-05-23T13:29:02Z | |
| dc.date.issued | 2025 | en |
| dc.description.abstract | We aimed to validate an in vitro model of proliferative vitreoretinopathy (PVR) and investigate the effect of 670 nm light photobiomodulation (PBM) on PVR pathophysiology.Immortalised retinal pigment epithelial cells (ARPE-19) were seeded at 3.0 × 103 cells/well. Half were stimulated with TGF-β1 to induce EMT, then all cells were allocated to either PBM, one treatment of 670 nm light every 3 hours for 12 hours or control. EMT morphology was characterised by area, perimeter, perimeter-area ratio, maximum Feret diameter, minimum Feret diameter, Feret ratio, compactness, eccentricity, and radius. The same parameters were analysed to determine the effect of PBM on EMT morphology.TGF-β induced morphological changes in ARPE-19 consistent with EMT, namely increased eccentricity, compactness, maximum Feret diameter, Feret ratio and perimeter-area ratio. Treatment with 670 nm PBM mitigates changes in perimeter and maximum Feret diameter induced by EMT.The TGF-β1 stimulation of RPE cells produces a morphologically valid in vitro model of EMT. PBM is effective at mitigating some EMT-related morphological changes. | en |
| dc.description.sponsorship | This work was funded by a charitable donation to Clear Vision Research, John Curtin School of Medical Research, ANU, to whom the authors give special thanks. | en |
| dc.description.status | Peer-reviewed | en |
| dc.format.extent | 6 | en |
| dc.identifier.issn | 0065-2598 | en |
| dc.identifier.other | PubMed:39930195 | en |
| dc.identifier.other | ORCID:/0000-0003-3086-2739/work/215746329 | en |
| dc.identifier.scopus | 85218438959 | en |
| dc.identifier.uri | http://www.scopus.com/inward/record.url?scp=85218438959&partnerID=8YFLogxK | en |
| dc.identifier.uri | https://hdl.handle.net/1885/733752399 | |
| dc.language.iso | en | en |
| dc.rights | © 2025. The Author(s) | en |
| dc.source | Advances in Experimental Medicine and Biology | en |
| dc.subject | ARPE-19 | en |
| dc.subject | CellProfiler | en |
| dc.subject | Epithelial-mesenchymal transition | en |
| dc.subject | Photobiomodulation (PBM) | en |
| dc.subject | Proliferative vitreoretinopathy | en |
| dc.subject | Rhegmatogenous retinal detachment | en |
| dc.subject | TGF-β | en |
| dc.title | Effect of Photobiomodulation on Proliferative Changes in the Retina: Evidence from an In Vitro Model of PVR | en |
| dc.type | Journal article | en |
| dspace.entity.type | Publication | en |
| local.bibliographicCitation.lastpage | 200 | en |
| local.bibliographicCitation.startpage | 195 | en |
| local.contributor.affiliation | Watson, Oscar; Australian National University Medical School | en |
| local.contributor.affiliation | Aggio-Bruce, Riemke; Australian National University Medical School | en |
| local.contributor.affiliation | Essex, Rohan; Medical School Directorate, School of Medicine and Psychology, ANU College of Science and Medicine, The Australian National University | en |
| local.contributor.affiliation | Valter, Krisztina; Australian National University Medical School | en |
| local.identifier.citationvolume | 1468 | en |
| local.identifier.doi | 10.1007/978-3-031-76550-6_32 | en |
| local.identifier.pure | f7e2a540-910a-40ee-8f89-7e070cf0763f | en |
| local.identifier.url | https://www.scopus.com/pages/publications/85218438959 | en |
| local.type.status | Published | en |