Around the World in 26 Million Years: Diversification and Biogeography of Pantropical Grass-Yellow <i>Eurema</i> Butterflies (Pieridae: Coliadinae)

Abstract

AimGrass-yellow butterflies (Eurema) are a group of pantropical Pieridae distributed throughout Asia, Australasia, Africa and the New World. However, little is known about their diversification, including the biogeographic mechanism(s) explaining their circumglobal distribution. We present the first densely sampled, time-calibrated phylogeny and biogeographic reconstruction of grass-yellows to confirm the monophyly of the genera, re-evaluate their taxonomy and infer the biogeographic events contributing to their worldwide distribution.LocationGlobal tropics and subtropics.TaxonThe butterfly tribe Euremini (Pieridae: Coliadinae).MethodsWe analysed up to 391 genetic loci from 126 samples of 66 ingroup species. Divergence dating was accomplished in a Bayesian phylogenetic framework using secondary calibration points, and maximum likelihood models of various biogeographic models were fitted to the data using the R package BioGeoBEARS. We used the best fitting model to estimate relative dispersal events with biogeographical stochastic mapping. Finally, we estimated branch-specific speciation and extinction rates to assess the diversification history of the group.ResultsDifferent phylogenomic analyses converged on similar topologies with robust support. Grass-yellows emerged ca. 26 Mya in the New World, and a single extant lineage dispersed to Asia in the early Miocene, where they diversified and dispersed to Africa and Australasia. The fastest rates of diversification occurred in the Old World tropics during the late Miocene. Many of the grass-yellow genera were either paraphyletic or polyphyletic as traditionally circumscribed. To maintain nomenclatural stability, we place all grass-yellows in Eurema sensu lato and recognise two subgenera: Eurema (Abaeis) and Eurema (Eurema).Main ConclusionsGrass-yellow butterflies originated in the Americas and attained their global distributional patterns via dispersal. The Indo-Australian and Caribbean archipelagoes seem to have accelerated the diversification of the group, and movement in and out of these island regions was frequent. Although the traditional view of founder-event speciation envisions migrants from large landmasses ('mainland') colonising smaller landmasses ('islands'), we find that island to mainland dispersal and differentiation were equally or more common.