Superanionic Solvent-Free Liquid Enzymes Exhibit Enhanced Structures and Activities

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dc.contributor.authorZhou, Yeen
dc.contributor.authorPedersen, Jannik Nedergaarden
dc.contributor.authorPedersen, Jacob Nedergaarden
dc.contributor.authorJones, Nykola C.en
dc.contributor.authorHoffmann, Søren Vrønningen
dc.contributor.authorPetersen, Steen Vangen
dc.contributor.authorPedersen, Jan Skoven
dc.contributor.authorPerriman, Adamen
dc.contributor.authorGao, Renjunen
dc.contributor.authorGuo, Zhengen
dc.date.accessioned2026-03-29T10:40:37Z
dc.date.available2026-03-29T10:40:37Z
dc.date.issued2022-08-21en
dc.description.abstractThe surface of a carboxylate-enriched octuple mutant of Bacillus subtilis lipase A (8M) is chemically anionized to produce core (8M)-shell (cationic polymer surfactants) bionanoconjugates in protein liquid form, which are termed anion-type biofluids. The resultant lipase biofluids exhibit a 2.5-fold increase in hydrolytic activity when compared with analogous lipase biofluids based on anionic polymer surfactants. In addition, the applicability of the anion-type biofluid using Myoglobin (Mb) that is well studied in anion-type solvent-free liquid proteins is evaluated. Although anionization resulted in the complete unfolding of Mb, the active α-helix level is partially recovered in the anion-type biofluids, and the effect is accentuated in the cation-type Mb biofluids. These highly active anion-type solvent-free liquid enzymes exhibit increased thermal stability and provide a new direction in solvent-free liquid protein research.en
dc.description.sponsorshipYe Zhou acknowledges the Chinese Scholarship Council (CSC) for the financial support for his study at Aarhus University. Financial Support from Novo Nordisk Foundation (NNF16OC0021740) and AUFF-NOVA (AUFF-E-2015-FLS-9- 12) were gratefully acknowledged. The authors thank ISA (C.B.) for the allocation of beam time on the AU-UV beamline on ASTRID2.en
dc.description.statusPeer-revieweden
dc.format.extent9en
dc.identifier.issn2198-3844en
dc.identifier.otherORCID:/0000-0003-2205-9364/work/209602508en
dc.identifier.scopus85136478266en
dc.identifier.urihttps://hdl.handle.net/1885/733808044
dc.language.isoenen
dc.provenanceThis is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.en
dc.rights© 2022 The Authors.en
dc.sourceAdvanced Scienceen
dc.subjectanionizationen
dc.subjectcationic polymer surfactanten
dc.subjectlipaseen
dc.subjectmyoglobinen
dc.subjectsolvent-free liquid enzymeen
dc.titleSuperanionic Solvent-Free Liquid Enzymes Exhibit Enhanced Structures and Activitiesen
dc.typeJournal articleen
dspace.entity.typePublicationen
local.contributor.affiliationZhou, Ye; Aarhus Universityen
local.contributor.affiliationPedersen, Jannik Nedergaard; Aarhus Universityen
local.contributor.affiliationPedersen, Jacob Nedergaard; Aarhus Universityen
local.contributor.affiliationJones, Nykola C.; Aarhus Universityen
local.contributor.affiliationHoffmann, Søren Vrønning; Aarhus Universityen
local.contributor.affiliationPetersen, Steen Vang; Aarhus Universityen
local.contributor.affiliationPedersen, Jan Skov; Aarhus Universityen
local.contributor.affiliationPerriman, Adam; University of Bristolen
local.contributor.affiliationGao, Renjun; Jilin Universityen
local.contributor.affiliationGuo, Zheng; Aarhus Universityen
local.identifier.citationvolume9en
local.identifier.doi10.1002/advs.202202359en
local.identifier.pure3469e202-d11e-431b-b58d-afa0ba1dd5b0en
local.identifier.urlhttps://www.scopus.com/pages/publications/85136478266en
local.type.statusPublisheden

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