A lanthanide tag for a complementary set of pseudocontact shifts
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Topping, Lydia
Welegedara, Adarshi P.
Judd, Martyna
Abdelkader, Elwy H.
Cox, Nicholas
Otting, Gottfried
Butler, Stephen J.
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Pseudocontact shifts (PCS) generated by paramagnetic lanthanide ions deliver powerful restraints for protein structure analysis by NMR spectroscopy. We present a new lanthanide tag that generates different PCSs than that of a related tag, which differs in structure by a single oxygen atom. It is highly reactive towards cysteine and performs well in turn-on luminescence and in EPR spectroscopy.We present a new lanthanide tag C14, which differs from our C12 tag by a single oxygen atom and generates distinct and complementary pseudocontact shifts, enhancing protein structure determination via paramagnetic NMR, EPR and emission spectroscopy.
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Chemical Communications
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