<i>Plasmodium vivax</i> malaria serological exposure markers: Assessing the degree and implications of cross-reactivity with <i>P. knowlesi</i>

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dc.contributor.authorLongley, Rhea J.en
dc.contributor.authorGrigg, Matthew J.en
dc.contributor.authorSchoffer, Kaelen
dc.contributor.authorObadia, Thomasen
dc.contributor.authorHyslop, Stephanieen
dc.contributor.authorPiera, Kim A.en
dc.contributor.authorNekkab, Narimaneen
dc.contributor.authorMazhari, Raminen
dc.contributor.authorTakashima, Eizoen
dc.contributor.authorTsuboi, Takafumien
dc.contributor.authorHarbers, Matthiasen
dc.contributor.authorTetteh, Kevinen
dc.contributor.authorDrakeley, Chrisen
dc.contributor.authorChitnis, Chetan E.en
dc.contributor.authorHealer, Julieen
dc.contributor.authorTham, Wai Hongen
dc.contributor.authorSattabongkot, Jetsumonen
dc.contributor.authorWhite, Michael T.en
dc.contributor.authorCooper, Daniel J.en
dc.contributor.authorRajahram, Giri S.en
dc.contributor.authorBarber, Bridget E.en
dc.contributor.authorWilliam, Timothyen
dc.contributor.authorAnstey, Nicholas M.en
dc.contributor.authorMueller, Ivoen
dc.date.accessioned2025-05-31T07:29:08Z
dc.date.available2025-05-31T07:29:08Z
dc.date.issued2022-05-26en
dc.description.abstractSerological markers are a promising tool for surveillance and targeted interventions for Plasmodium vivax malaria. P. vivax is closely related to the zoonotic parasite P. knowlesi, which also infects humans. P. vivax and P. knowlesi are co-endemic across much of South East Asia, making it important to design serological markers that minimize cross-reactivity in this region. To determine the degree of IgG cross-reactivity against a panel of P. vivax serological markers, we assayed samples from human patients with P. knowlesi malaria. IgG antibody reactivity is high against P. vivax proteins with high sequence identity with their P. knowlesi ortholog. IgG reactivity peaks at 7 days post-P. knowlesi infection and is short-lived, with minimal responses 1 year post-infection. We designed a panel of eight P. vivax proteins with low levels of cross-reactivity with P. knowlesi. This panel can accurately classify recent P. vivax infections while reducing misclassification of recent P. knowlesi infections.en
dc.description.sponsorshipWe acknowledge the efforts of the original study teams for collecting the P. knowlesi clinical samples. We thank the following organisations for donation of malaria-naive control plasma: the Australian and Thai Red Cross; the Rio de Janeiro State Blood Bank (with support from Andre M. Siqueira); and the Volunteer Blood Donor Registry at WEHI. We thank Shazia Ruybal for support generating an automated quality control platform through R, based on prior work by Connie Li-Wai-Suen, which was also used for the standard curve conversion. We thank Sarah Miller for assistance with ethical approvals. We would like to thank the Director-General, Ministry of Health, Malaysia, for permission to publish this manuscript. We also acknowledge the Victorian State Government Operational Infrastructure Support and Australian Government NHMRC IRIISS. Funding: WEHI Innovation Fund (R.L. I.M.). Clinical Trials Funding: Malaysian Ministry of Health (grant number BP00500420), the Asia Pacific Malaria Elimination Network (108-07), and the Australian National Health and Medical Research Council (NHMRC; 1037304, 1045156, 115680). NHMRC Fellowships to N.M.A. #1135820, M.J.G. #1138860, and R.L. #1173210. NHMRC grants #1092789, #1134989, #1132975 and #1043345 (I.M.). M.J.G. was also supported by the Australian Centre for International Agricultural Research (Grant# LS-2019-116). Conceptualization: R.L. M.G. N.A. and I.M. Methodology \u2013 clinical trials & control samples: R.L. M.G. K.P. J.S. G.R. B.B. D.C. T.W. N.A. and I.M. Methodology \u2013 protein expression: E.T. T.T. M.H. C.E.C. J.H. and W.H.T. Methodology \u2013 antibody assays & analytics: R.L. K.S. R.M. N.N. T.O. and M.W. Investigation \u2013 laboratory: R.L. K.S. S.H. and R.M. Investigation \u2013 analytics: R.L. K.S. T.O. and M.W. Visualization: R.L. K.S. T.O. and M.W. Supervision: N.A. and I.M. Writing \u2013 original draft: R.L. Writing \u2013 reviewing & editing: R.L. M.G. K.S. S.H. K.P. N.N. T.O. R.M. E.T. T.T. M.H. C.E.C. D.C. G.R. K.T. C.D. J.H. W.H.T. J.S. M.W. B.B. T.W. N.A. and T.M. R.L. M.W. T.T. and and I.M. are inventors on filed patent PCT/US17/67926 on a system, method, apparatus, and diagnostic test for P. vivax. M.H. was an employee of the company CellFree Sciences Co. Ltd. While citing references scientifically relevant for this work, we also actively worked to promote gender balance in our reference list. The author list of this paper includes contributors from the location where the research was conducted who participated in the data collection, design, analysis, and/or interpretation of the work. We acknowledge the efforts of the original study teams for collecting the P. knowlesi clinical samples. We thank the following organisations for donation of malaria-naive control plasma: the Australian and Thai Red Cross; the Rio de Janeiro State Blood Bank (with support from Andre M. Siqueira); and the Volunteer Blood Donor Registry at WEHI. We thank Shazia Ruybal for support generating an automated quality control platform through R, based on prior work by Connie Li-Wai-Suen, which was also used for the standard curve conversion. We thank Sarah Miller for assistance with ethical approvals. We would like to thank the Director-General, Ministry of Health, Malaysia, for permission to publish this manuscript. We also acknowledge the Victorian State Government Operational Infrastructure Support and Australian Government NHMRC IRIISS. Funding: WEHI Innovation Fund (R.L., I.M.). Clinical Trials Funding: Malaysian Ministry of Health (grant number BP00500420 ), the Asia Pacific Malaria Elimination Network ( 108-07 ), and the Australian National Health and Medical Research Council (NHMRC; 1037304 , 1045156 , 115680 ). NHMRC Fellowships to N.M.A. #1135820 , M.J.G. #1138860 , and R.L. #1173210 . NHMRC grants #1092789 , #1134989 , #1132975 and #1043345 (I.M.). M.J.G. was also supported by the Australian Centre for International Agricultural Research (Grant# LS-2019-116 ).en
dc.description.statusPeer-revieweden
dc.format.extent13en
dc.identifier.otherPubMed:35732155en
dc.identifier.scopus85132610519en
dc.identifier.urihttp://www.scopus.com/inward/record.url?scp=85132610519&partnerID=8YFLogxKen
dc.identifier.urihttps://hdl.handle.net/1885/733756186
dc.language.isoenen
dc.rightsPublisher Copyright: © 2022 The Authorsen
dc.sourceCell Reports Medicineen
dc.subjectantibodiesen
dc.subjectantibody cross-reactivityen
dc.subjectmalariaen
dc.subjectmalaria eliminationen
dc.subjectPlasmodium knowlesien
dc.subjectPlasmodium vivaxen
dc.subjectserological exposure markersen
dc.subjectserosurveillanceen
dc.subjectspecies cross-reactivityen
dc.title<i>Plasmodium vivax</i> malaria serological exposure markers: Assessing the degree and implications of cross-reactivity with <i>P. knowlesi</i>en
dc.typeJournal articleen
dspace.entity.typePublicationen
local.contributor.affiliationLongley, Rhea J.; Walter and Eliza Hall Institute of Medical Researchen
local.contributor.affiliationGrigg, Matthew J.; Charles Darwin Universityen
local.contributor.affiliationSchoffer, Kael; Walter and Eliza Hall Institute of Medical Researchen
local.contributor.affiliationObadia, Thomas; Université Paris Citéen
local.contributor.affiliationHyslop, Stephanie; Walter and Eliza Hall Institute of Medical Researchen
local.contributor.affiliationPiera, Kim A.; Charles Darwin Universityen
local.contributor.affiliationNekkab, Narimane; Université Paris Citéen
local.contributor.affiliationMazhari, Ramin; Walter and Eliza Hall Institute of Medical Researchen
local.contributor.affiliationTakashima, Eizo; Ehime Universityen
local.contributor.affiliationTsuboi, Takafumi; Ehime Universityen
local.contributor.affiliationHarbers, Matthias; Ltd.en
local.contributor.affiliationTetteh, Kevin; London School of Hygiene and Tropical Medicineen
local.contributor.affiliationDrakeley, Chris; London School of Hygiene and Tropical Medicineen
local.contributor.affiliationChitnis, Chetan E.; Institut Pasteur Parisen
local.contributor.affiliationHealer, Julie; Walter and Eliza Hall Institute of Medical Researchen
local.contributor.affiliationTham, Wai Hong; Walter and Eliza Hall Institute of Medical Researchen
local.contributor.affiliationSattabongkot, Jetsumon; Mahidol Universityen
local.contributor.affiliationWhite, Michael T.; Université Paris Citéen
local.contributor.affiliationCooper, Daniel J.; Charles Darwin Universityen
local.contributor.affiliationRajahram, Giri S.; Clinical Research Centreen
local.contributor.affiliationBarber, Bridget E.; Charles Darwin Universityen
local.contributor.affiliationWilliam, Timothy; Infectious Diseases Society Kota Kinabalu Sabahen
local.contributor.affiliationAnstey, Nicholas M.; Charles Darwin Universityen
local.contributor.affiliationMueller, Ivo; Walter and Eliza Hall Institute of Medical Researchen
local.identifier.citationvolume3en
local.identifier.doi10.1016/j.xcrm.2022.100662en
local.identifier.pureee4217e5-a924-4086-9b65-0bf3304b703fen
local.identifier.urlhttps://www.scopus.com/pages/publications/85132610519en
local.type.statusPublisheden

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