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Optimal functional imaging of dendritic activity via oblique single-photon excitation of voltage indicators

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Date

2020

Authors

Castanares, Michael
Bachor, Hans A.
Daria, Vincent

Journal Title

Journal ISSN

Volume Title

Publisher

SPIE

Access Statement

Research Projects

Organizational Units

Journal Issue

Abstract

We use patterned light to initiate scattered photons that are obliquely directed to excite voltage indicators, which report changes in the membrane potential. Our proposed scheme reduces the excitation of voltage indicators elsewhere in the cell that constitutes the background signal and therefore enables us to amplify the detected fluorescence carrying the voltage information.

Description

Keywords

Functional voltage imaging, Holographic projection, Two-photon imaging

Citation

URI

https://hdl.handle.net/1885/733799112

Collections

ANU Research Publications

Source

Type

Conference paper

Book Title

Biomedical Imaging and Sensing Conference 2020

Entity type

Publication

Access Statement

License Rights

DOI

10.1117/12.2573217

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